General Information of This Photoreceptor (PR)
PR ID PR00085
PR Name Improved Light-Inducible Dimer (iLID)
Source of Species Avena sativa
Class LOV domain
UniProt ID O49003
GenBank 747155673
PDB ID 4WF0
Sequence
LATTLERIEKNFIITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRFLQGPETDRATV
RKIRDAIDNQTEVTVQLINYTKSGKKFWNVFHLQPMRDYKGDVQYFIGVQLDGTERLHGA
AEREAVCLIKKTAFQIAEAANDEYNF
Components Photoreceptor Improved Light-Inducible Dimer (iLID)
Complementary Protein Stringent starvation protein B (SspB)
Detail Info
Cofactor Flavin mononucleotide (FMN)
Detail Info
3D Structure
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Complementary Protein Detail of This PR
Complementary Protein Stringent starvation protein B (SspB)
UniProt ID P0AFZ3
Sequence
MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAV
GNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDE
EASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK
Cofactor Detail of This PR
Cofactor Name Flavin mononucleotide (FMN)
PubChem CID 643976
Structure
Formula C17H21N4O9P
Molecular Weight 456.3
Optogenetic System (OS) Consisting of This PR
OS00182
OS Info
OS Name: Light regulated signal protein localization and activity system 1 (LRPLA 1)
[1]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Stringent starvation protein B
OS00183
OS Info
OS Name: Light regulated signal protein localization and activity system 2 (LRPLA 2)
[1]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Stringent starvation protein B
OS00190
OS Info
OS Name: Light-induceble tethering system (LIT)
[2]
Recruited protein: Cytochrome b5
Fusion protein: OMM linker peptide sequence
OS00648
OS Info
OS Name: Opto-dHTH, an optogenetic inhibitor to completely disrupt GqGTP-PLC interactions 1 (Opto-dHTH 1)
[3]
Fusion protein: Helix-turn-helix (HTH) region of the PLC3
Recruited protein: Stringent starvation protein B
OS00649
OS Info
OS Name: Opto-dHTH, an optogenetic inhibitor to completely disrupt GqGTP-PLC interactions 2 (Opto-dHTH 2)
[3]
Fusion protein: Helix-turn-helix (HTH) region of the PLC3
Recruited protein: Stringent starvation protein B
OS00650
OS Info
OS Name: Opto-dHTH, an optogenetic inhibitor to completely disrupt GqGTP-PLC interactions 3 (Opto-dHTH 3)
[3]
Fusion protein: Helix-turn-helix (HTH) region of the PLC3 (I860A, 27-mer)
Recruited protein: Nuclear export signal
OS00651
OS Info
OS Name: Opto-dHTH, an optogenetic inhibitor to completely disrupt GqGTP-PLC interactions 4 (Opto-dHTH 4)
[3]
Recruited protein: Cytochrome b5
Fusion protein: Helix-turn-helix (HTH) region of the PLC3 (27-mer)
OS00652
OS Info
OS Name: Opto-dHTH, an optogenetic inhibitor to completely disrupt GqGTP-PLC interactions 5 (Opto-dHTH 5)
[3]
Recruited protein: Helix-turn-helix (HTH) region of the PLC3 (I860A, 27-mer)
Functional protein: Improved Light-Inducible Dimer
OS00193
OS Info
OS Name: Optogenetic control Cdc42 system (Opto-Cdc42)
[4]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Intersectin-1
OS00623
OS Info
OS Name: Optogenetic control Exoc70 for membrane traffiking system 2 (Opto-Exoc70-MT 2)
[5]
Recruited protein: Exocyst complex component 70
Functional protein: Improved Light-Inducible Dimer
OS00192
OS Info
OS Name: Optogenetic control Rac system (Opto-Rac)
[4]
Recruited protein: Endogenous Rac1 protein
Functional protein: Improved Light-Inducible Dimer
OS00191
OS Info
OS Name: Optogenetic control RhoA system (Opto-RhoA)
[6] , [7]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Rho guanine nucleotide exchange factor 12
OS00630
OS Info
OS Name: Optogenetic mitochondira deformation system 2 (OptoMD 2)
[8]
Recruited protein: Kinesin heavy chain isoform 5A
Fusion protein: Transmembrane domain of Miro1
OS00631
OS Info
OS Name: Optogenetic mitochondira deformation system 3 (OptoMD 3)
[8]
Recruited protein: Kinesin-1 heavy chain
Fusion protein: Transmembrane domain of Miro1
OS00186
OS Info
OS Name: Optogenetic recuit factors to microtubule plus ends system 1 (Opto-RFMT 1)
[9]
Fusion protein: EB-binding SxIP motif
Recruited protein: Nuclear export signal
OS00187
OS Info
OS Name: Optogenetic recuit factors to microtubule plus ends system 2 (Opto-RFMT 2)
[9]
Fusion protein: EB-binding SxIP motif
Recruited protein: Nuclear export signal
OS00188
OS Info
OS Name: Optogenetic recuit factors to microtubule plus ends system 3 (Opto-RFMT 3)
[9]
Fusion protein: EB-binding SxIP motif
Recruited protein: Nuclear export signal
OS00189
OS Info
OS Name: Optogenetic recuit factors to microtubule plus ends system 4 (Opto-RFMT 4)
[9]
Fusion protein: EB-binding SxIP motif
Recruited protein: Nuclear export signal
OS00184
OS Info
OS Name: Optogenetic regulate iTrkA activity by iLID system (Opto-iTrkA-iLID)
[10]
Recruited protein: Stringent starvation protein B
Fusion protein: Tropomyosin receptor kinases A
OS00185
OS Info
OS Name: Optogenetic regulate iTrkB activity by iLID system (Opto-iTrkB-iLID)
[10]
Fusion protein: Intracellular Tropomyosin receptor kinase B
Recruited protein: Stringent starvation protein B
OS00710
OS Info
OS Name: Optogenetics of LOV domains with chemiluminescence 2 (Opto-chemilu 2)
[11]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Stringent starvation protein B
OS00178
OS Info
OS Name: Optogentic protein recruitment by iLID system 1 (OptoPR-iLID 1)
[12]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Stringent starvation protein B
OS00179
OS Info
OS Name: Optogentic protein recruitment by iLID system 2 (OptoPR-iLID 2)
[12]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Stringent starvation protein B
OS00180
OS Info
OS Name: Optogentic protein recruitment by iLID system 3 (OptoPR-iLID 3)
[12]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Stringent starvation protein B
OS00181
OS Info
OS Name: Optogentic protein recruitment by iLID system 4 (OptoPR-iLID 4)
[12]
Functional protein: Improved Light-Inducible Dimer
Recruited protein: Stringent starvation protein B
OS00177
OS Info
OS Name: Optogentic regulate stromal interaction molecule 1 system 5 (OptoSTIM1-5)
[13]
Recruited protein: Stringent starvation protein B
Fusion protein: Stromal interaction molecule 1
References
1 Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins
2 Optogenetic Control of Endoplasmic Reticulum-Mitochondria Tethering
3 Spatiotemporal Optical Control of Gq-PLC Interactions
4 Cells lay their own tracks - optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration
5 An active tethering mechanism controls the fate of vesicles
6 Optogenetic control of small GTPases reveals RhoA mediates intracellular calcium signaling
7 Increasing spatial resolution of photoregulated GTPases through immobilized peripheral membrane proteins
8 Light-inducible deformation of mitochondria in live cells
9 Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker
10 Construction of Light-Activated Neurotrophin Receptors Using the Improved Light-Induced Dimerizer (iLID)
11 Photoactivation of LOV domains with chemiluminescence
12 Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment
13 Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling