Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00186
OS Name
Optogenetic recuit factors to microtubule plus ends system 1 (Opto-RFMT 1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Improved Light-Inducible Dimer (iLID)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
747155673
PDB ID
4WF0
3D Structure
Components
Photoreceptor: Improved Light-Inducible Dimer (iLID)
Complementary Protein: Stringent starvation protein B (SspB)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Stringent starvation protein B (SspB)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein EB-binding SxIP motif
Detail Info
Recruited protein Nuclear export signal
Detail Info
Stringent starvation protein B
Detail Info
OS Application Information (1)
Mechanism of Action ILID comformational change and subsequently iLID-SspB heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application SxIP-improved light-dinducible dimer for facilitating the reversible recruitment of factors to microtubule plus ends
Light Information Activation Wavelength 488 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Minutes
Light Intensity 0.15 milliwatts
Expression Information Expression Method Transfection
Cell Lines S2 cells
References
1 Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker