Details of the Optogenetic System
General Information of This Optogenetic System (OS) | ||||||
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OS ID |
OS00192
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OS Name |
Optogenetic control Rac system
(Opto-Rac)
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[1] | ||||
Photoreceptor (PR) |
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Complementary Protein | Stringent starvation protein B (SspB) | |||||
Cofactor | Flavin mononucleotide (FMN) | |||||
Controlled Protein | Recruited protein | Endogenous Rac1 protein | ||||
Functional protein | Improved Light-Inducible Dimer | |||||
OS Application Information (1) | ||||||
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Mechanism of Action | ILID comformational change and subsequently iLID-SspB heterodimerization | [ 1] | ||||
Controlled Signal Pathway | Rac1 activation | |||||
Effect | Sensor | |||||
Application | Optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration | |||||
Note | Not need exogenous fibronectin yet require integrin binding and myosin contractility | |||||
Light Information | Activation Wavelength | 467 nm | ||||
Activation Time | Seconds | |||||
Expression Information | Expression Method | Transfection | ||||
Cell Lines | Mouse IA32 fibroblasts | |||||