Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00650
OS Name
Opto-dHTH, an optogenetic inhibitor to completely disrupt GqGTP-PLC interactions 3 (Opto-dHTH 3)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Improved Light-Inducible Dimer (iLID)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
747155673
PDB ID
4WF0
3D Structure
Components
Photoreceptor: Improved Light-Inducible Dimer (iLID)
Complementary Protein: Stringent starvation protein B (SspB)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Stringent starvation protein B (SspB)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Fusion protein Helix-turn-helix (HTH) region of the PLC3 (I860A, 27-mer)
Detail Info
Recruited protein Nuclear export signal
Detail Info
OS Application Information (1)
Mechanism of Action iLID-SspB heterodimerization [ 1]
Controlled Signal Pathway Gq-PLC signaling
Effect Inhibition
Application Disrupt GqGTP-PLC interactions reversibly, control PIP2 hydrolysis
Light Information Activation Wavelength Blue
Process Reversible
Expression Information Expression Method Transfection
Cell Lines HeLa cells
References
1 Spatiotemporal Optical Control of Gq-PLC Interactions