Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00180
|
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| OS Name |
Optogentic protein recruitment by iLID system 3
(OptoPR-iLID 3)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Stringent starvation protein B (SspB) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Functional protein | Improved Light-Inducible Dimer | ||||
| Recruited protein | Stringent starvation protein B | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | ILID comformational change and subsequently iLID-SspB heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Protein translocation | |||||
| Effect | Sensor | |||||
| Application | Local optogenetic protein recruitment by fusing iLID with different membrane locator protein | |||||
| Light Information | Activation Wavelength | 445 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Seconds | |||||
| Deactivation Time | Seconds | |||||
| Expression Information | Expression Method | Transduction | ||||
| Cell Lines | HEK 293T cells | |||||