Details of the Controlled Protein | OPTICS

General Information of This Controlled Protein (CP)
CP ID	CP00424
CP Name	Stringent starvation protein B (SspB)
CP Type	Recruited protein
Common Name	Stringent starvation protein B (Adapter protein SspB) (Specificity-enhancing factor SspB)
Organism	Escherichia coli (strain K12)
UniProt ID	P0AFZ3
UniProt Entry	SSPB_ECOLI
KEGG	ecj:JW3197; eco:b3228
Function	Enhances recognition of ssrA-tagged proteins by the ClpX-ClpP protease; the ssrA degradation tag (AANDENYALAA) is added trans-translationally to proteins that are stalled on the ribosome, freeing the ribosome and targeting stalled peptides for degradation. SspB activates the ATPase activity of ClpX. Seems to act in concert with SspA in the regulation of several proteins during exponential and stationary-phase growth.; Also stimulates degradation of the N-terminus of RseA (residues 1-108, alone or in complex with sigma-E) by ClpX-ClpP in a non-ssrA-mediated fashion.
Sequence	MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAV GNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDE EASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK

Optogenetic System (OS) Controlling This CP

OS00182

OS Name: Light regulated signal protein localization and activity system 1; [1]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00183

OS Name: Light regulated signal protein localization and activity system 2; [1]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00204

OS Name: Optical dimerizar ssrA-cpLOV2; [2]
Photoreceptor (PR) Name: LOV2 domain of circular permutants (I539E) (cpLOV2 (I539E)); PR Info
Components: Photoreceptor; LOV2 domain of circular permutants (I539E) (cpLOV2 (I539E))
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00648

OS Name: Opto-dHTH, an optogenetic inhibitor to completely disrupt GqGTP-PLC interactions 1; [3]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00186

OS Name: Optogenetic recuit factors to microtubule plus ends system 1; [4]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00187

OS Name: Optogenetic recuit factors to microtubule plus ends system 2; [4]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00188

OS Name: Optogenetic recuit factors to microtubule plus ends system 3; [4]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00189

OS Name: Optogenetic recuit factors to microtubule plus ends system 4; [4]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00178

OS Name: Optogentic protein recruitment by iLID system 1; [5]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00179

OS Name: Optogentic protein recruitment by iLID system 2; [5]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00180

OS Name: Optogentic protein recruitment by iLID system 3; [5]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00181

OS Name: Optogentic protein recruitment by iLID system 4; [5]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

OS00177

OS Name: Optogentic regulate stromal interaction molecule 1 system 5; [6]
Photoreceptor (PR) Name: Improved Light-Inducible Dimer (iLID); PR Info
Components: Photoreceptor; Improved Light-Inducible Dimer (iLID)
: Complementary Protein; Stringent starvation protein B (SspB)
: Cofactor; Flavin mononucleotide (FMN)

References

1	Engineering an improved light-induced dimer (iLID) for controlling the localization and activity of signaling proteins
2	Circularly permuted LOV2 as a modular photoswitch for optogenetic engineering
3	Spatiotemporal Optical Control of Gq-PLC Interactions
4	Control of microtubule dynamics using an optogenetic microtubule plus end-F-actin cross-linker
5	Optimized iLID Membrane Anchors for Local Optogenetic Protein Recruitment
6	Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling