Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00193
|
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| OS Name |
Optogenetic control Cdc42 system
(Opto-Cdc42)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Stringent starvation protein B (SspB) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Functional protein | Improved Light-Inducible Dimer | ||||
| Recruited protein | Intersectin-1 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | ILID comformational change and subsequently iLID-SspB heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Cdc42 activation | |||||
| Effect | Sensor | |||||
| Application | Optogenetic Cdc42 activation stimulates fibronectin deposition supporting directed migration | |||||
| Note | Not need exogenous fibronectin yet require integrin binding and myosin contractility | |||||
| Light Information | Activation Wavelength | 467 nm | ||||
| Activation Time | Seconds | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Mouse IA32 fibroblasts | |||||