Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00652
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| OS Name |
Opto-dHTH, an optogenetic inhibitor to completely disrupt GqGTP-PLC interactions 5
(Opto-dHTH 5)
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[1] | ||||
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Photoreceptor (PR) |
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| Complementary Protein | Stringent starvation protein B (SspB) | |||||
| Cofactor | Flavin mononucleotide (FMN) | |||||
| Controlled Protein | Recruited protein | Helix-turn-helix (HTH) region of the PLC3 (I860A, 27-mer) | ||||
| Functional protein | Improved Light-Inducible Dimer | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | iLID-SspB heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Gq-PLC signaling | |||||
| Effect | Inhibition | |||||
| Application | Disrupt GqGTP-PLC interactions reversibly, control PIP2 hydrolysis | |||||
| Note | Prevent the diffusion of activated iLID to unintended regions | |||||
| Light Information | Activation Wavelength | Blue | ||||
| Process | Reversible | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HeLa cells | |||||