Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00630
OS Name	Optogenetic mitochondira deformation system 2 (OptoMD 2)		[1]
Photoreceptor (PR) PR Info	PR Name Improved Light-Inducible Dimer (iLID) Source of Species Avena sativa Class LOV domain UniProt ID O49003 GenBank 747155673 PDB ID 4WF0 3D Structure Components Photoreceptor: Improved Light-Inducible Dimer (iLID) Complementary Protein: Stringent starvation protein B (SspB) Cofactor: Flavin mononucleotide (FMN)
Complementary Protein	Stringent starvation protein B (SspB)
Cofactor	Flavin mononucleotide (FMN)
Controlled Protein	Recruited protein	Kinesin heavy chain isoform 5A	Detail Info
Controlled Protein	Fusion protein	Transmembrane domain of Miro1	Detail Info

OS Application Information (1)
Mechanism of Action	iLID-SspB heterodimerization		[ 1]
Controlled Signal Pathway	Mitochondria deformation
Effect	Activation
Application	Light inducible deformation of mitochondria by recruiting molecular motors to the outer mitochrondrial membrane
Note	Both the outer and inner membranes of mitochondria can be stretched and deformed using this optogenetic system
Light Information	Activation Wavelength	Blue
	Process	Reversible
	Deactivation Wavelength	Dark
	Activation Time	10 s
	Deactivation Time	Minutes
	Light Intensity	9.7 watts per square centimeter
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	COS cells; HeLa cells; U2OS cells; 3T3 cells

References

Light-inducible deformation of mitochondria in live cells