Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00007
PR Name Cryptochrome 2 (CRY2)
Source of Species Arabidopsis thalino
Class Cryptochromes
UniProt ID Q96524
GenBank NP_849588
PDB ID 6K8I
Sequence
MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQFYPGRASRWWMKQSL
AHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYDPVSLVRDHTVKEKLV
ERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIESVMLPPPWRLMPITAA
AEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNEFIEKQLIDYAKNSKKVV
GNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEESADLFLRGIGLREYSRYI
CFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMRELWATGWMHNRIRV
IVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYISGSIPDGHELDRLDNPALQG
AKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKPIVDIDTAREL
LAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKEPGLCPSVSSNDQQVPSAVRYNGS
KRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSVFFVSQSCSLASEGKNLEGIQDSSD
QITTSLGKNGCK
Components Photoreceptor Cryptochrome 2 (CRY2)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Detail Info
Cofactor Flavin adenine dinucleotide (FAD)
Detail Info
3D Structure
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Complementary Protein Detail of This PR
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
UniProt ID A0A061DZ54
Sequence
MNRALPEMLQCSDMTVLERQRARLKWQQEQLQQQQLQQQEQQQSYFSELSGVFSSQPSHV
EGFQGGLMSGDSVLGDMVMTRQLKPDPGLETAWPELVKVDMPGMGFGPCGYGNGPSFDMN
YAISRTSSCPPAVAAAVAGEVVEVKGKESVVSEKIGSAVGRESFKKRKVDKLQNLKVVAE
DDSKRIKACAEEGESKITGPNTNKSSSNNNNNKKESSTDTSKENSKVSEVQKPDYIHVRA
RRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEF
LSMKLAAVNPRLDFNVENLFAKEVFPSCTTNFPTVGMSSEMANPPYLQVSPVQHVVSCCG
LEMGMNTPDMAPRRTISAPVSIPDASFLDSSCFPQIQPSATWDVELQNLYNVAFDQGRST
SFPSQPFTGSIEASNLKMEM
Cofactor Detail of This PR
Cofactor Name Flavin adenine dinucleotide (FAD)
PubChem CID 643975
Structure
Formula C27H33N9O15P2
Molecular Weight 785.5
Optogenetic System (OS) Consisting of This PR
OS00058
OS Info
OS Name: Blue light control cell-cell interaction system (BLCC)
[1]
Recruited protein: Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein: Platelet-derived growth factor receptor
Detail Info
OS00055
OS Info
OS Name: Brainbow-based live imaging system (LOLlbow)
[2]
Fusion protein: CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
Recruited protein: CreN means N terminal of Cre recombinase and CreC means C terminal of Cre recombinase
Detail Info
OS00056
OS Info
OS Name: Exosomes for protein loading via optically reversible proteinCprotein interactions system (EXPLORs)
[3]
Functional protein: Cryptochrome-2
Detail Info
Recruited protein: Exosome-associated tetraspanin protein CD9
Detail Info
OS00050
OS Info
OS Name: Light switchable cellCcell interaction system (LSCCIS)
[4]
Functional protein: Cryptochrome-2
Detail Info
Recruited protein: Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
OS00054
OS Info
OS Name: Light-activated CRISPR-Cas9 (LACE)
[5]
Recruited protein: CRISPR-associated endonuclease Cas9
Detail Info
Fusion protein: LexA activation domain
Detail Info
OS00051
OS Info
OS Name: Light-controlled ADRB2 Intracellular trafficking system (LADRBIT)
[6]
Fusion protein: Beta-arrestin-2
Detail Info
Recruited protein: Beta2-adrenergic receptor
Detail Info
OS00053
OS Info
OS Name: Light-triggered modulation of K+ conductance system in mitochondria (LTMK+ in Mito)
[7]
Fusion protein: Regulatory protein GAL4
Detail Info
Recruited protein: Virus protein 16
Detail Info
OS00730
OS Info
OS Name: Optogenetic activation of LRP6 system (OptoLRP6)
[8] , [9]
Recruited protein: Transmembrane protein 198
Detail Info
Fusion protein: Wnt coreceptor LRP6
Detail Info
OS00061
OS Info
OS Name: Optogenetic BAX system (OptoBax)
[10]
Fusion protein: BCL2-associated X apoptosis regulator
Detail Info
Recruited protein: Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
OS00057
OS Info
OS Name: Optogenetic control LexA for transcription regulation system 1 (OptoLexA 1)
[11]
Fusion protein: Transcription regulator LexA
Detail Info
Recruited protein: Virus protein 16
Detail Info
OS00705
OS Info
OS Name: Optogenetic controling ventromedial orbitofrontal cortex system 1 (Opto-vmOFC 1)
[12] , [13]
Functional protein: Channelrhodopsin 2
Detail Info
OS00067
OS Info
OS Name: Optogenetic endomembrane targeting toolbox (OpEn-Tag)
[14]
Recruited protein: Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein: EB-binding SxIP motif
Detail Info
OS00627
OS Info
OS Name: Optogenetic Modulate intraocular pressure system (OptoMIP)
[15]
Recruited protein: CAAX prenyl protease?
Detail Info
Fusion protein: Inositol-polyphosphate-5-phosphatase OCRL
Detail Info
OS00062
OS Info
OS Name: Optogenetic PKA system 1 (OptoPKA 1)
[10]
Fusion protein: A cAMP dependent protein kinase holoenzyme
Detail Info
Recruited protein: Mitochondrial import receptor subunit TOM20
Detail Info
OS00063
OS Info
OS Name: Optogenetic PKA system 2 (OptoPKA 2)
[10]
Fusion protein: A cAMP dependent protein kinase holoenzyme
Detail Info
Recruited protein: Mitochondrial import receptor subunit TOM20
Detail Info
OS00064
OS Info
OS Name: Optogenetic PKA system 3 (OptoPKA 3)
[10]
Fusion protein: A cAMP dependent protein kinase holoenzyme
Detail Info
Recruited protein: Mitochondrial import receptor subunit TOM20
Detail Info
OS00065
OS Info
OS Name: Optogenetic PKA system 4 (OptoPKA 4)
[10]
Fusion protein: A cAMP dependent protein kinase holoenzyme
Detail Info
Recruited protein: Mitochondrial import receptor subunit TOM20
Detail Info
OS00066
OS Info
OS Name: Optogenetic PKA system 5 (OptoPKA 5)
[10]
Fusion protein: A cAMP dependent protein kinase holoenzyme
Detail Info
Recruited protein: Mitochondrial import receptor subunit TOM20
Detail Info
OS00060
OS Info
OS Name: Optogentic regulate stromal interaction molecule 1 system 2 (OptoSTIM1-2)
[16]
Fusion protein: Stromal interaction molecule 1
Detail Info
OS00664
OS Info
OS Name: P21-CRY2/CIB1 system 1 (Opto-CRY2/CIB1 1)
[17]
Fusion protein: CDK-interacting protein p21 (CDKN1A)-nuclear localization signal (Arg 140, Lys 141 and Arg 142 replaced by Ala)
Detail Info
OS00665
OS Info
OS Name: P21-CRY2/CIB1 system 2 (Opto-CRY2/CIB1 2)
[17]
Fusion protein: CDK-interacting protein p21 (CDKN1A)-nuclear localization signal (Arg 140, Lys 141 and Arg 142 replaced by Ala)
Detail Info
OS00666
OS Info
OS Name: P21-CRY2/CIB1 system 3 (Opto-CRY2/CIB1 3)
[17]
Fusion protein: CDK-interacting protein p21 (CDKN1A)-nuclear localization signal (Arg 140, Lys 141 and Arg 142 replaced by Ala)
Detail Info
OS00059
OS Info
OS Name: Photoactivatable GAL promoter to control transcription system 3 (PA-GAL 3)
[18]
Fusion protein: Regulatory protein GAL4
Detail Info
Recruited protein: Regulatory protein Gal4
Detail Info
OS00052
OS Info
OS Name: Photoactivatable Gal4 for transcription activation by Cryptochrome 2 (PA-Gal4-CRY2)
[19]
Fusion protein: Regulatory protein GAL4
Detail Info
Recruited protein: Virus protein 16
Detail Info
References
1 Independent Control over Multiple Cell Types in Space and Time Using Orthogonal Blue and Red Light Switchable Cell Interactions
2 Live imaging of multicolor-labeled cells in Drosophila
3 Exosome engineering for efficient intracellular delivery of soluble proteins using optically reversible protein-protein interaction module
4 Blue Light Switchable Cell-Cell Interactions Provide Reversible and Spatiotemporal Control Towards Bottom-Up Tissue Engineering
5 A light-inducible CRISPR-Cas9 system for control of endogenous gene activation
6 Unique Roles of -Arrestin in GPCR Trafficking Revealed by Photoinducible Dimerizers
7 Light-Regulated Transcription of a Mitochondrial-Targeted K(+) Channel
8 Activation of Wnt/-catenin pathway mitigates blood-brain barrier dysfunction in Alzheimer's disease
9 Optogenetic Control of the Canonical Wnt Signaling Pathway During Xenopus laevis Embryonic Development
10 Design, construction, and validation of optogenetic proteins
11 Benchmarking of optical dimerizer systems
12 Postnatal Phencyclidine-Induced Deficits in Decision Making Are Ameliorated by Optogenetic Inhibition of Ventromedial Orbitofrontal Cortical Glutamate Neurons
13 The Translational Utility of Circuit-Based Manipulations in Preclinical Models
14 OpEn-Tag-A Customizable Optogenetic Toolbox To Dissect Subcellular Signaling
15 Optogenetic Modulation of Intraocular Pressure in a Glucocorticoid-Induced Ocular Hypertension Mouse Model
16 Optogenetic manipulation of calcium signals in single T cells in vivo
17 Cell Cycle Control by Optogenetically Regulated Cell Cycle Inhibitor Protein p21
18 Real-time optogenetic control of intracellular protein concentration in microbial cell cultures
19 Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2