Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00051
|
|||||
| OS Name |
Light-controlled ADRB2 Intracellular trafficking system
(LADRBIT)
|
[1] | ||||
|
Photoreceptor (PR) |
|
|||||
| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | Beta-arrestin-2 | ||||
| Recruited protein | Beta2-adrenergic receptor | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIB1 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | ADRB1 intracellular trafficking | |||||
| Effect | Sensor | |||||
| Application | Analysis of intracellular trafficking of ADRB2 regulated by beta-arrestin | |||||
| Light Information | Activation Wavelength | 440 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 30 min | |||||
| Deactivation Time | 30 min | |||||
| Light Intensity | 3 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HEK293 cells | |||||