Details of the Controlled Protein

General Information of This Controlled Protein (CP)
CP ID CP00415
CP Name Virus protein 16 (VP16)
CP Type Recruited protein
Common Name Tegument protein VP16 (Alpha trans-inducing protein) (Alpha-TIF) (ICP25) (Vmw65)
Organism Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
UniProt ID P06492
UniProt Entry VP16_HHV11
KEGG vg:24271473
Function Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection.; May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.
Sequence
MDLLVDELFADMNADGASPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAA
LFNRLLDDLGFSAGPALCTMLDTWNEDLFSALPTNADLYRECKFLSTLPSDVVEWGDAYV
PERTQIDIRAHGDVAFPTLPATRDGLGLYYEALSRFFHAELRAREESYRTVLANFCSALY
RYLRASVRQLHRQAHMRGRDRDLGEMLRATIADRYYRETARLARVLFLHLYLFLTREILW
AAYAEQMMRPDLFDCLCCDLESWRQLAGLFQPFMFVNGALTVRGVPIEARRLRELNHIRE
HLNLPLVRSAATEEPGAPLTTPPTLHGNQARASGYFMVLIRAKLDSYSSFTTSPSEAVMR
EHAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSL
GDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFT
DALGIDEYGG
Optogenetic System (OS) Controlling This CP
OS00618
OS Info
OS Name
Fungal lightCoxygenCvoltage system carrying Hap1p DBD
[1]
Photoreceptor (PR) Name
Vivid PAS domain (VVD)
PR Info
Components
Photoreceptor
Vivid PAS domain (VVD)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00565
OS Info
OS Name
Irreversible Photoactivated Gal4 trancription system
[2], [3], [4]
Photoreceptor (PR) Name
Flavin Kelch-repeat F-box 1 (FKF1)
PR Info
Components
Photoreceptor
Flavin Kelch-repeat F-box 1 (FKF1)
Complementary Protein
GIGANTEA (GI)
Cofactor
Flavin mononucleotide (FMN)
OS00057
OS Info
OS Name
Optogenetic control LexA for transcription regulation system 1
[5]
Photoreceptor (PR) Name
Cryptochrome 2 (CRY2)
PR Info
Components
Photoreceptor
Cryptochrome 2 (CRY2)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00537
OS Info
OS Name
Photoactivable Gal4 for gene expression control system
[6]
Photoreceptor (PR) Name
Residue1-650 of Phytochrome B (PhyB (1-650))
PR Info
Components
Photoreceptor
Residue1-650 of Phytochrome B (PhyB (1-650))
Complementary Protein
Phytochrome interacting factor 6 (PIF6)
Cofactor
Phycocyanobilin (PCB)
OS00566
OS Info
OS Name
Photoactivable Gal4 for gene expression controlled by truncated PhyB system
[7]
Photoreceptor (PR) Name
Residue 1-450 of Phytochrome B (PhyB (1-450))
PR Info
Components
Photoreceptor
Residue 1-450 of Phytochrome B (PhyB (1-450))
Complementary Protein
Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor
Phycocyanobilin (PCB)
OS00052
OS Info
OS Name
Photoactivatable Gal4 for transcription activation by Cryptochrome 2
[8]
Photoreceptor (PR) Name
Cryptochrome 2 (CRY2)
PR Info
Components
Photoreceptor
Cryptochrome 2 (CRY2)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00518
OS Info
OS Name
Photoactivated GAL4 by BphP1 system
[9]
Photoreceptor (PR) Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
PR Info
Components
Photoreceptor
Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein
Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor
Biliverdin (BV)
OS00521
OS Info
OS Name
Photoactivated TetR by BphP1 system 1
[9]
Photoreceptor (PR) Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
PR Info
Components
Photoreceptor
Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein
Transcriptional regulator PpsR2 (PpsR2)
Cofactor
Biliverdin (BV)
OS00517
OS Info
OS Name
Photoactivated TetR by BphP1 system 2
[9]
Photoreceptor (PR) Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
PR Info
Components
Photoreceptor
Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein
Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor
Biliverdin (BV)
References
1 Modular and Molecular Optimization of a LOV (Light-Oxygen-Voltage)-Based Optogenetic Switch in Yeast
2 Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1
3 Induction of protein-protein interactions in live cells using light
4 FKF1 and GIGANTEA complex formation is required for day-length measurement in Arabidopsis
5 Benchmarking of optical dimerizer systems
6 PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants
7 Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B
8 Bidirectional approaches for optogenetic regulation of gene expression in mammalian cells using Arabidopsis cryptochrome 2
9 Near-infrared optogenetic pair for protein regulation and spectral multiplexing