Details of the Controlled Protein

Optogenetic manipulation of calcium signals in single T cells in vivo

Optogenetic control of endogenous Ca(2+) channels in vivo

Visual quantification of prostaglandin E(2) discharge from a single cell

Calcium transients trigger switch-like discharge of prostaglandin E2in an extracellular signal-regulated kinase-dependent manner

Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling

Non-invasive optical control of endogenous Ca(2+) channels in awake mice

General Information of This Controlled Protein (CP)
CP ID	CP00322
CP Name	Stromal interaction molecule 1 (STIM1)
CP Type	Fusion protein
Common Name	Stromal interaction molecule 1
Organism	Homo sapiens (Human)
UniProt ID	Q13586
UniProt Entry	STIM1_HUMAN
KEGG	hsa:6786
Function	Plays a role in mediating store-operated Ca(2+) entry (SOCE), a Ca(2+) influx following depletion of intracellular Ca(2+) stores. Acts as Ca(2+) sensor in the endoplasmic reticulum via its EF-hand domain. Upon Ca(2+) depletion, translocates from the endoplasmic reticulum to the plasma membrane where it activates the Ca(2+) release-activated Ca(2+) (CRAC) channel subunit ORAI1. Involved in enamel formation. Activated following interaction with STIMATE, leading to promote STIM1 conformational switch.
Sequence	MDVCVRLALWLLWGLLLHQGQSLSHSHSEKATGTSSGANSEESTAAEFCRIDKPLCHSED EKLSFEAVRNIHKLMDDDANGDVDVEESDEFLREDLNYHDPTVKHSTFHGEDKLISVEDL WKAWKSSEVYNWTVDEVVQWLITYVELPQYEETFRKLQLSGHAMPRLAVTNTTMTGTVLK MTDRSHRQKLQLKALDTVLFGPPLLTRHNHLKDFMLVVSIVIGVGGCWFAYIQNRYSKEH MKKMMKDLEGLHRAEQSLHDLQERLHKAQEEHRTVEVEKVHLEKKLRDEINLAKQEAQRL KELREGTENERSRQKYAEEELEQVREALRKAEKELESHSSWYAPEALQKWLQLTHEVEVQ YYNIKKQNAEKQLLVAKEGAEKIKKKRNTLFGTFHVAHSSSLDDVDHKILTAKQALSEVT AALRERLHRWQQIEILCGFQIVNNPGIHSLVAALNIDPSWMGSTRPNPAHFIMTDDVDDM DEEIVSPLSMQSPSLQSSVRQRLTEPQHGLGSQRDLTHSDSESSLHMSDRQRVAPKPPQM SRAADEALNAMTSNGSHRLIEGVHPGSLVEKLPDSPALAKKALLALNHGLDKAHSLMELS PSAPPGGSPHLDSSRSHSPSSPDPDTPSPVGDSRALQASRNTRIPHLAGKKAVAEEDNGS IGEETDSSPGRKKFPLKIFKKPLKK

Optogenetic System (OS) Controlling This CP

OS00073

OS Name: Enhenced optogentic regulate stromal interaction molecule 1 system 1; [1]
Photoreceptor (PR) Name: Photolyase homology region of Arabidopsis Cryptochromes 2 (D387A) (CRY2PHR (D387A)); PR Info
Components: Photoreceptor; Photolyase homology region of Arabidopsis Cryptochromes 2 (D387A) (CRY2PHR (D387A))
: Complementary Protein; Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
: Cofactor; Flavin adenine dinucleotide (FAD)

OS00070

OS Name: Enhenced optogentic regulate stromal interaction molecule 1 system 2; [1]
Photoreceptor (PR) Name: Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G)); PR Info
Components: Photoreceptor; Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G))
: Complementary Protein; Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
: Cofactor; Flavin adenine dinucleotide (FAD)

OS00071

OS Name: Enhenced optogentic regulate stromal interaction molecule 1 system 3; [1]
Photoreceptor (PR) Name: Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G)); PR Info
Components: Photoreceptor; Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G))
: Complementary Protein; Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
: Cofactor; Flavin adenine dinucleotide (FAD)

OS00036

OS Name: Optogentic regulate stromal interaction molecule 1 system 1; [2], [3], [4]
Photoreceptor (PR) Name: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR); PR Info
Components: Photoreceptor; Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
: Complementary Protein; Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
: Cofactor; Flavin adenine dinucleotide (FAD)

OS00060

OS Name: Optogentic regulate stromal interaction molecule 1 system 2; [1]
Photoreceptor (PR) Name: Cryptochrome 2 (CRY2); PR Info
Components: Photoreceptor; Cryptochrome 2 (CRY2)
: Complementary Protein; Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
: Cofactor; Flavin adenine dinucleotide (FAD)

OS00038

OS Name: Optogentic regulate stromal interaction molecule 1 system 4; [5]
Photoreceptor (PR) Name: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR); PR Info
Components: Photoreceptor; Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
: Complementary Protein; Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
: Cofactor; Flavin adenine dinucleotide (FAD)

OS00177

OS00568

References