Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00054
|
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| OS Name |
Light-activated CRISPR-Cas9
(LACE)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | CRISPR-associated endonuclease Cas9 | ||||
| Fusion protein | LexA activation domain | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2PHRCCIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Transcription activation | |||||
| Effect | Activation | |||||
| Application | Endogenous gene activation for CRISPR-Cas 9 | |||||
| Note | Greater flexibility and simplicity in targeting any endogenous gene | |||||
| Light Information | Activation Wavelength | 450 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Light Intensity | 16 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HEK293T cells | |||||