Details of the Controlled Protein

General Information of This Controlled Protein (CP)
CP ID CP00414
CP Name Regulatory protein Gal4 (Gal4AD)
CP Type Recruited protein
Common Name Regulatory protein GAL4
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
UniProt ID P04386
UniProt Entry GAL4_YEAST
KEGG sce:YPL248C
Function This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
Sequence
MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESR
LERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPL
TLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDFMPRDALHGFDWSEEDDM
SDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVNRLPTMITDRYTLASRSTT
SRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQILFNCILAIGAWCIEGESTD
IDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQKTNTSYNFHSFSIRMAISLG
LNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRSIQLSQNTISFPSSVDDVQRTT
TGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICAKKCLMICNEIEEVSRQAPKFLQ
MDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVLRDFFTNFTQKKSQLEQDQNDHQS
YEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAWNCSYYLFNAVLVPIKTLLSNSKSN
AENNETAQLLQQINTVLMLLKKLATFKIQTCEKYIQVLEEVCAPFLLSQCAIPLPHISYN
NSNGSAIKNIVGSATIAQYPTLPEENVNNISVKYVSPGSVGPSPVPLKSGASFSDLVKLL
SNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQLQSLVPLTPSALFGGANFNQSGNIADSS
LSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGW
TDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE
Optogenetic System (OS) Controlling This CP
OS00520
OS Info
OS Name
Near-infrared light-controlled gene expression system
[1], [2]
Photoreceptor (PR) Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
PR Info
Components
Photoreceptor
Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein
Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor
Biliverdin (BV)
OS00150
OS Info
OS Name
Optogenetic control gene expression by two LOV blue-light photoreceptor system
[3]
Photoreceptor (PR) Name
Vivid PAS domain (VVD)
PR Info
Components
Photoreceptor
Vivid PAS domain (VVD)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00515
OS Info
OS Name
Photoactivatable GAL promoter to control transcription
[4]
Photoreceptor (PR) Name
Phytochrome B (PhyB)
PR Info
Components
Photoreceptor
Phytochrome B (PhyB)
Complementary Protein
Phytochrome interacting factor 3 (PIF3)
Cofactor
Phycocyanobilin (PCB)
OS00031
OS Info
OS Name
Photoactivatable GAL promoter to control transcription system 2
[]
Photoreceptor (PR) Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
PR Info
Components
Photoreceptor
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00059
OS Info
OS Name
Photoactivatable GAL promoter to control transcription system 3
[5]
Photoreceptor (PR) Name
Cryptochrome 2 (CRY2)
PR Info
Components
Photoreceptor
Cryptochrome 2 (CRY2)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00518
OS Info
OS Name
Photoactivated GAL4 by BphP1 system
[6]
Photoreceptor (PR) Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
PR Info
Components
Photoreceptor
Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein
Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor
Biliverdin (BV)
References
1 Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing
2 Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells
3 Fungal Light-Oxygen-Voltage Domains for Optogenetic Control of Gene Expression and Flocculation in Yeast
4 Light-mediated control of DNA transcription in yeast
5 Real-time optogenetic control of intracellular protein concentration in microbial cell cultures
6 Near-infrared optogenetic pair for protein regulation and spectral multiplexing