Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00730
|
|||||
| OS Name |
Optogenetic activation of LRP6 system
(OptoLRP6)
|
[1], [2] | ||||
|
Photoreceptor (PR) |
|
|||||
| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Recruited protein | Transmembrane protein 198 | ||||
| Fusion protein | Wnt coreceptor LRP6 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heteromerization | [ 1] , [ 2] | ||||
| Controlled Signal Pathway | Wnt/-catenin pathway | |||||
| Effect | Activation | |||||
| Application | Mitigates blood-brain barrier dysfunction in Alzheimer's disease | |||||
| Light Information | Activation Wavelength | 450 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 20min | |||||
| Deactivation Time | 40 min | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Mice brain endothelial cells | |||||