Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00060
|
|||||
| OS Name |
Optogentic regulate stromal interaction molecule 1 system 2
(OptoSTIM1-2)
|
[1] | ||||
|
Photoreceptor (PR) |
|
|||||
| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | Stromal interaction molecule 1 | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2 homo-oligomerization | [ 1] | ||||
| Controlled Signal Pathway | CRAC channel activation and Ca2+ influx | |||||
| Effect | Activation | |||||
| Application | Control calcium signals in single T cell in vivo | |||||
| Light Information | Activation Wavelength | 470 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Seconds to Minutes | |||||
| Deactivation Time | 10 min | |||||
| Light Intensity | 710 milliwatts | |||||
| Expression Information | Expression Method | Transduction | ||||
| Cell Lines | T cells and CD8+ T cells | |||||