Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00067
OS Name
Optogenetic endomembrane targeting toolbox (OpEn-Tag)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Cryptochrome 2 (CRY2)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6K8I
3D Structure
Components
Photoreceptor: Cryptochrome 2 (CRY2)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Cryptochrome-interacting basic-helix-loop-helix 1
Detail Info
Fusion protein EB-binding SxIP motif
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Activation
Application A verstile tool to dissect subcellular signaling
Note CIBN attachs to organelles while CRY2 attach to specific proteins for different cell signal regulated
Light Information Activation Wavelength 450 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Within 10 min
Light Intensity 1.33 watts per square meter
Expression Information Expression Method Transfection and Transduction
Cell Lines Hela cells
References
1 OpEn-Tag-A Customizable Optogenetic Toolbox To Dissect Subcellular Signaling