Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00050
|
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| OS Name |
Light switchable cellCcell interaction system
(LSCCIS)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Functional protein | Cryptochrome-2 | ||||
| Recruited protein | Cryptochrome-interacting basic-helix-loop-helix 1 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Cell-cell interaction | |||||
| Effect | Sensor | |||||
| Application | Noninvasively control cell-cell interaction with light | |||||
| Note | A new way to study cell-cell interaction and assembling cellular building blocks into tissues with unmatched flexibility | |||||
| Light Information | Activation Wavelength | 471 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | Minutes | |||||
| Deactivation Time | Minutes | |||||
| Light Intensity | 80 microwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | MDA-MB-231 cells | |||||