Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00618
OS Name
Fungal lightCoxygenCvoltage system carrying Hap1p DBD (HAP-LOV)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Vivid PAS domain (VVD)
Source of Species
Neurospora crassa
Class
LOV domain
UniProt ID
Q9C3Y6
GenBank
149243087
PDB ID
2PDR
3D Structure
Components
Photoreceptor: Vivid PAS domain (VVD)
Cofactor: Flavin adenine dinucleotide (FAD)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Heme-responsive zinc finger transcription factor HAP1
Detail Info
Recruited protein Transcription factor p65
Detail Info
Virus protein 16
Detail Info
OS Application Information (1)
Mechanism of Action VVD homodimerization [ 1]
Controlled Signal Pathway Gene expression regulation
Effect Activation
Application LOV(LightCOxygenCVoltage)-Based gene expression regulation
Note Hap1p DBD performed better than using Gal4 DBD as DNA binding domain to control gene expression in VVD mediated optogenetic sysytem
Light Information Activation Wavelength 466 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 30 min
Deactivation Time 120 min
Light Intensity 24 micromole per square meter per second
Expression Information Expression Method Transformation
Cell Lines Yeast
References
1 Modular and Molecular Optimization of a LOV (Light-Oxygen-Voltage)-Based Optogenetic Switch in Yeast