Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00517
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| OS Name |
Photoactivated TetR by BphP1 system 2
(PA-TetR-BphP1-2)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1) | |||||
| Cofactor | Biliverdin (BV) | |||||
| Controlled Protein | Fusion protein | Tetracycline repressor protein class D | ||||
| Recruited protein | Virus protein 16 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | BphP1-Q-PAS1 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Transcription activation | |||||
| Effect | Activation | |||||
| Application | Activate gene expression by recruit TetR to nucleus | |||||
| Note | Q-PAS1 is three fold smaller than PpsR2 and lack oligomerization thus more efficient to control transcription than Bph1-PpsR2 | |||||
| Light Information | Activation Wavelength | 740 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 30 s | |||||
| Deactivation Time | 180 s | |||||
| Light Intensity | 0.2 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Hela cells | |||||