Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00518
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| OS Name |
Photoactivated GAL4 by BphP1 system
(PA-GAL4-BphP1)
|
[1] | ||||
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Photoreceptor (PR) |
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| Complementary Protein | Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1) | |||||
| Cofactor | Biliverdin (BV) | |||||
| Controlled Protein | Functional protein | Bacterial phytochrome photoreceptors 1 | ||||
| Recruited protein | Regulatory protein Gal4 | |||||
| Virus protein 16 | ||||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | BphP1-Q-PAS1 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | GAL4 translocation to inhibit gene expression | |||||
| Effect | Inhibition | |||||
| Application | Transcription inhibition by Q-PAS1 relocalization to plasma membrane and disruption of DNA binding | |||||
| Note | Q-PAS1 is three fold smaller than PpsR2 and lack oligomerization | |||||
| Light Information | Activation Wavelength | 740 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 30 s | |||||
| Deactivation Time | 180 s | |||||
| Light Intensity | 0.2 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Hela cells | |||||