Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00565
OS Name	Irreversible Photoactivated Gal4 trancription system (IR-PA-Gal4)		[1], [2], [3]
Photoreceptor (PR) PR Info	PR Name Flavin Kelch-repeat F-box 1 (FKF1) Source of Species Arabiodopsis Class LOV domain UniProt ID D7KVS5 GenBank AKX66784.1 3D Structure Components Photoreceptor: Flavin Kelch-repeat F-box 1 (FKF1) Complementary Protein: GIGANTEA (GI) Cofactor: Flavin mononucleotide (FMN)
Complementary Protein	GIGANTEA (GI)
Cofactor	Flavin mononucleotide (FMN)
Controlled Protein	Fusion protein	Regulatory protein GAL4	Detail Info
Controlled Protein	Recruited protein	Virus protein 16	Detail Info

OS Application Information (1)
Mechanism of Action	FKF1-GI heterodimerization		[ 1] , [ 2] , [ 3]
Controlled Signal Pathway	Transcription activation
Effect	Activation
Application	Optogenetic control transcription in mammalian cells
Note	A long-lived transcriptional response to a single pulse of light and extremely low back-ground activity in the dark
Light Information	Activation Wavelength	447.5 nm
	Process	Ireversible
	Activation Time	8 s
	Light Intensity	6.25 microwatts per square millimeter
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	HEK293 cells and NIH 3T3 cells

References

1	Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1
2	Induction of protein-protein interactions in live cells using light
3	FKF1 and GIGANTEA complex formation is required for day-length measurement in Arabidopsis