Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00537
OS Name	Photoactivable Gal4 for gene expression control system (PA-Gal4)		[1]
Photoreceptor (PR) PR Info	PR Name Residue1-650 of Phytochrome B (PhyB (1-650)) Source of Species Arabidopsis Class Phytochromes UniProt ID P14713 GenBank NP_179469 PDB ID 4OUR 3D Structure Components Photoreceptor: Residue1-650 of Phytochrome B (PhyB (1-650)) Complementary Protein: Phytochrome interacting factor 6 (PIF6) Cofactor: Phycocyanobilin (PCB)
Complementary Protein	Phytochrome interacting factor 6 (PIF6)
Cofactor	Phycocyanobilin (PCB)
Controlled Protein	Fusion protein	Regulatory protein GAL4	Detail Info
Controlled Protein	Recruited protein	Virus protein 16	Detail Info

OS Application Information (1)
Mechanism of Action	PhyB-PIF6 heterodimerization		[ 1]
Controlled Signal Pathway	Gene expression activation
Effect	Activation
Application	A versatile optoswitch for spatiotemporal control of gene expression in Drosophila explants
Note	A valuable resource to investigate complex biological; developmental and pathological processes in ex vivo paradigms
Light Information	Activation Wavelength	630 nm
	Process	Reversible
	Deactivation Wavelength	720 nm
	Activation Time	Hours
	Deactivation Time	10 s
	Light Intensity	0.75 milliwatts per square centimeter
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	Drosophila S2R+ cells

References

PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants