Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00306
PR Name Residue1-650 of Phytochrome B (PhyB (1-650))
Source of Species Arabidopsis
Class Phytochromes
UniProt ID P14713
GenBank NP_179469
PDB ID 4OUR
Sequence
MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQ
YTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCM
IAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFV
AREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVR
AISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIG
LHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQ
YMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEF
LMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAA
FLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMA
VAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWE
TAEMDAIHSLQLILRDSFKESEAAMNSKVVDGVVQPCRDMAGEQGIDELG
Components Photoreceptor Residue1-650 of Phytochrome B (PhyB (1-650))
Complementary Protein Phytochrome interacting factor 6 (PIF6)
Detail Info
Cofactor Phycocyanobilin (PCB)
Detail Info
3D Structure
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Complementary Protein Detail of This PR
Complementary Protein Phytochrome interacting factor 6 (PIF6)
UniProt ID Q8L5W7
Sequence
MMFLPTDYCCRLSDQEYMELVFENGQILAKGQRSNVSLHNQRTKSIMDLYEAEYNEDFMK
SIIHGGGGAITNLGDTQVVPQSHVAAAHETNMLESNKHVDDSETLKASSSKRMMVDYHNR
KKIKFIPPDEQSVVADRSFKLGFDTSSVGFTEDSEGSMYLSSSLDDESDDARPQVPARTR
KALVKRKRNAEAYNSPERNQRNDINKKMRTLQNLLPNSHKDDNESMLDEAINYMTNLQLQ
VQMMTMGNRFVTPSMMMPLGPNYSQMGLAMGVGMQMGEQQFLPAHVLGAGLPGINDSADM
LRFLNHPGLMPMQNSAPFIPTENCSPQSVPPSCAAFPNQIPNPNSLSNLDGATLHKKSRK
TNR
Cofactor Detail of This PR
Cofactor Name Phycocyanobilin (PCB)
PubChem CID 5280816
Structure
Formula C33H38N4O6
Molecular Weight 586.7
Optogenetic System (OS) Consisting of This PR
OS00542
OS Info
OS Name: Optogenetic bind and release biomolecules trap (Opto-trap)
[1]
Functional protein: Phytochrome B
Detail Info
Recruited protein: Phytochrome interacting factor 3
Detail Info
OS00538
OS Info
OS Name: Optogenetic intracellular transport system 2 (Opto-IT 2)
[2]
Recruited protein: Kinesin-1 heavy chain
Detail Info
Fusion protein: Ras-related protein Rab-11A
Detail Info
OS00539
OS Info
OS Name: Optogenetic intracellular transport system 4 (Opto-IT 4)
[2]
Recruited protein: Kinesin-like protein KIF1A
Detail Info
Fusion protein: Ras-related protein Rab-11A
Detail Info
OS00540
OS Info
OS Name: Optogenetic intracellular transport system 6 (Opto-IT 6)
[2]
Recruited protein: Protein bicaudal D homolog 2
Detail Info
Fusion protein: Ras-related protein Rab-11A
Detail Info
OS00541
OS Info
OS Name: Optogenetic-drived affinity purification system (Opto-DAP)
[3]
Functional protein: Phytochrome B
Detail Info
Recruited protein: Tyrosine-protein kinase ZAP-70
Detail Info
OS00537
OS Info
OS Name: Photoactivable Gal4 for gene expression control system (PA-Gal4)
[4]
Fusion protein: Regulatory protein GAL4
Detail Info
Recruited protein: Virus protein 16
Detail Info
References
1 Generic and reversible opto-trapping of biomolecules
2 A Phytochrome-Derived Photoswitch for Intracellular Transport
3 Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome
4 PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants