Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00566
OS Name
Photoactivable Gal4 for gene expression controlled by truncated PhyB system (PA-Gal4-TrPhyB)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue 1-450 of Phytochrome B (PhyB (1-450))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Residue 1-450 of Phytochrome B (PhyB (1-450))
Complementary Protein: Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor Phycocyanobilin (PCB)
Controlled Protein Fusion protein Regulatory protein GAL4
Detail Info
Recruited protein Virus protein 16
Detail Info
Virus protein 16
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF6 heterodimerization [ 1]
Controlled Signal Pathway Transcription activation
Effect Activation
Application Truncated phytochrome for transcriptional regulation
Note Truncated PhyB and PIF6 can control transcription with lower invasiveness
Light Information Activation Wavelength 660 nm
Process Reversible
Deactivation Wavelength 735 nm
Activation Time 5 min
Deactivation Time 5 min
Light Intensity 0.1-0.45 milliwatts per square centimeter (660 nm); 0.12-0.5 milliwatts per square centimeter (735 nm)
Expression Information Expression Method Transfection
Cell Lines HEK293 cells
References
1 Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B