Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00521
OS Name
Photoactivated TetR by BphP1 system 1 (PA-TetR-BphP1-1)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
Source of Species
Rhodopseudomonas palustris
Class
Phytochromes
UniProt ID
A0A161I5N6
GenBank
1569047741
PDB ID
5OY5
3D Structure
Components
Photoreceptor: Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein: Transcriptional regulator PpsR2 (PpsR2)
Cofactor: Biliverdin (BV)
Complementary Protein Transcriptional regulator PpsR2 (PpsR2)
Cofactor Biliverdin (BV)
Controlled Protein Fusion protein Tetracycline repressor protein class D
Detail Info
Recruited protein Virus protein 16
Detail Info
OS Application Information (1)
Mechanism of Action BphP1-PpsR2 heterodimerization [ 1]
Controlled Signal Pathway Transcription activation
Effect Activation
Application Activate gene expression by recruit TetR to nucleus
Light Information Activation Wavelength 740 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 30 s
Deactivation Time 180 s
Light Intensity 0.2 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 Near-infrared optogenetic pair for protein regulation and spectral multiplexing