Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00323
PR Name Flavin Kelch-repeat F-box 1 (FKF1)
Source of Species Arabiodopsis
Class LOV domain
UniProt ID D7KVS5
GenBank AKX66784.1
Sequence
MARKHATESIGEVTGKRKKRDRVEEEGDIYDGIENQDVVEDGKLPLEVGMVYYPVTPPSF
IVSDALEPDFPLIYVNKVFEVFTGYHADEVLGRNCRFLQYRDPRAQRRHPLVDPVVVSEI
RRCLEEGIEFQGELLNFRKDGTPLVNRLRLALIRDDDGTVTHVIGIQVFSETTIDLDRVS
YPVFKHKQQLDQTSECLLPGGSPRFKEHHEDFCGILQMSDEVLAHNILSRLTPRDVASIG
SACRRLRQLTKNESVRKMVCQNAWGKEITGTLEIMTKKLGWGRLARELTTLEAVCWRKFT
VGGIVQPSRCNFSACAVGNRLVLFGGEGVNMQPLDDTFVLNLDAEYPEWQRVRVTSSPPG
RWGHTLSCLNGSWLVVFGGCGRQGLLNDVFVLDLDAKHPTWKEVAGGTPPLPRSWHSSCT
IEGSKLVVSGGCTDAGVLLSDTFLLDLTTDKPTWKEIPTSWAPPSRLGHSLSVFGRTKIL
MFGGLANSGHLKLRSGEAYTIDLEDEEPRWRELECSSFTGAVVPPPRLDHVAVSMPCGRV
IIFGGSIAGLHSPSQLFLIDPAEEKPSWRILNVPGKPPKLAWGHSTCVVGGTRVLVLGGH
TGEEWILNELHELCLASRQDSDL
Components Photoreceptor Flavin Kelch-repeat F-box 1 (FKF1)
Complementary Protein GIGANTEA (GI)
Detail Info
Cofactor Flavin mononucleotide (FMN)
Detail Info
3D Structure
Click to Save PDB File
Complementary Protein Detail of This PR
Complementary Protein GIGANTEA (GI)
UniProt ID Q9SQI2
Sequence
MASSSSSERWIDGLQFSSLLWPPPRDPQQHKDQVVAYVEYFGQFTSEQFPDDIAELVRHQ
YPSTEKRLLDDVLAMFVLHHPEHGHAVILPIISCLIDGSLVYSKEAHPFASFISLVCPSS
ENDYSEQWALACGEILRILTHYNRPIYKTEQQNGDTERNCLSKATTSGSPTSEPKAGSPT
QHERKPLRPLSPWISDILLAAPLGIRSDYFRWCSGVMGKYAAGELKPPTIASRGSGKHPQ
LMPSTPRWAVANGAGVILSVCDDEVARYETATLTAVAVPALLLPPPTTSLDEHLVAGLPA
LEPYARLFHRYYAIATPSATQRLLLGLLEAPPSWAPDALDAAVQLVELLRAAEDYASGVR
LPRNWMHLHFLRAIGIAMSMRAGVAADAAAALLFRILSQPALLFPPLSQVEGVEIQHAPI
GGYSSNYRKQIEVPAAEATIEATAQGIASMLCAHGPEVEWRICTIWEAAYGLIPLNSSAV
DLPEIIVATPLQPPILSWNLYIPLLKVLEYLPRGSPSEACLMKIFVATVETILSRTFPPE
SSRELTRKARSSFTTRSATKNLAMSELRAMVHALFLESCAGVELASRLLFVVLTVCVSHE
AQSSGSKRPRSEYASTTENIEANQPVSNNQTANRKSRNVKGQGPVAAFDSYVLAAVCALA
CEVQLYPMISGGGNFSNSAVAGTITKPVKINGSSKEYGAGIDSAISHTRRILAILEALFS
LKPSSVGTPWSYSSSEIVAAAMVAAHISELFRRSKALTHALSGLMRCKWDKEIHKRASSL
YNLIDVHSKVVASIVDKAEPLEAYLKNTPVQKDSVTCLNWKQENTCASTTCFDTAVTSAS
RTEMNPRGNHKYARHSDEGSGRPSEKGIKDFLLDASDLANFLTADRLAGFYCGTQKLLRS
VLAEKPELSFSVVSLLWHKLIAAPEIQPTAESTSAQQGWRQVVDALCNVVSATPAKAAAA
VVLQAERELQPWIAKDDEEGQKMWKINQRIVKVLVELMRNHDRPESLVILASASDLLLRA
TDGMLVDGEACTLPQLELLEATARAIQPVLAWGPSGLAVVDGLSNLLKCRLPATIRCLSH
PSAHVRALSTSVLRDIMNQSSIPIKVTPKLPTTEKNGMNSPSYRFFNAASIDWKADIQNC
LNWEAHSLLSTTMPTQFLDTAARELGCTISLSQ
Cofactor Detail of This PR
Cofactor Name Flavin mononucleotide (FMN)
PubChem CID 643976
Structure
Formula C17H21N4O9P
Molecular Weight 456.3
Optogenetic System (OS) Consisting of This PR
OS00565
OS Info
OS Name: Irreversible Photoactivated Gal4 trancription system (IR-PA-Gal4)
[1] , [2] , [3]
Fusion protein: Regulatory protein GAL4
Detail Info
Recruited protein: Virus protein 16
Detail Info
References
1 Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1
2 Induction of protein-protein interactions in live cells using light
3 FKF1 and GIGANTEA complex formation is required for day-length measurement in Arabidopsis