Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00298
PR Name Phytochrome B (PhyB)
Source of Species Arabidopsis
Class Phytochromes
UniProt ID P14713
GenBank NP_179469
PDB ID 4OUR
Sequence
MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQ
YTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCM
IAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFV
AREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVR
AISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIG
LHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQ
YMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEF
LMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAA
FLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMA
VAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWE
TAEMDAIHSLQLILRDSFKES
Components Photoreceptor Phytochrome B (PhyB)
Complementary Protein Phytochrome interacting factor 3 (PIF3)
Detail Info
Cofactor Phycocyanobilin (PCB)
Detail Info
3D Structure
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Complementary Protein Detail of This PR
Complementary Protein Phytochrome interacting factor 3 (PIF3)
UniProt ID O80536
Sequence
MPLFELFRLTKAKLESAQDRNPSPPVDEVVELVWENGQISTQSQSSRSRNIPPPQANSSR
AREIGNGSKTTMVDEIPMSVPSLMTGLSQDDDFVPWLNHHPSLDGYCSDFLRDVSSPVTV
NEQESDMAVNQTAFPLFQRRKDGNESAPAASSSQYNGFQSHSLYGSDRARDLPSQQTNPD
RFTQTQEPLITSNKPSLVNFSHFLRPATFAKTTNNNLHDTKEKSPQSPPNVFQTRVLGAK
DSEDKVLNESVASATPKDNQKACLISEDSCRKDQESEKAVVCSSVGSGNSLDGPSESPSL
SLKRKHSNIQDIDCHSEDVEEESGDGRKEAGPSRTGLGSKRSRSAEVHNLSERRRRDRIN
EKMRALQELIPNCNKVDKASMLDEAIEYLKSLQLQVQIMSMASGYYLPPAVMFPPGMGHY
PAAAAAMAMGMGMPYAMGLPDLSRGGSSVNHGPQFQVSGMQQQPVAMGIPRVSGGGIFAG
SSTIGNGSTRDLSGSKDQTTTNNNSNLKPIKRKQGSSDQFCGSS
Cofactor Detail of This PR
Cofactor Name Phycocyanobilin (PCB)
PubChem CID 5280816
Structure
Formula C33H38N4O6
Molecular Weight 586.7
Optogenetic System (OS) Consisting of This PR
OS00514
OS Info
OS Name: Optogenetic controlled SOS signaling system (OptoSOS)
[1]
Recruited protein: Nucleotide exchange factor SOS
Detail Info
Functional protein: Phytochrome B
Detail Info
OS00515
OS Info
OS Name: Photoactivatable GAL promoter to control transcription (PA-GAL)
[2]
Fusion protein: Regulatory protein GAL4
Detail Info
Recruited protein: Regulatory protein Gal4
Detail Info
References
1 Multichromatic Control of Signaling Pathways in Mammalian Cells
2 Light-mediated control of DNA transcription in yeast