Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00515
|
|||||
| OS Name |
Photoactivatable GAL promoter to control transcription
(PA-GAL)
|
[1] | ||||
|
Photoreceptor (PR) |
||||||
| Complementary Protein | Phytochrome interacting factor 3 (PIF3) | |||||
| Cofactor | Phycocyanobilin (PCB) | |||||
| Controlled Protein | Fusion protein | Regulatory protein GAL4 | ||||
| Recruited protein | Regulatory protein Gal4 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | PhyB-PIF3 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | DNA transcription | |||||
| Effect | Activation | |||||
| Application | Control DNA transcription | |||||
| Light Information | Activation Wavelength | 660 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | 730 nm | |||||
| Activation Time | 5 s | |||||
| Deactivation Time | Seconds | |||||
| Light Intensity | 2.8 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | Yeast | |||||