Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00150
OS Name
Optogenetic control gene expression by two LOV blue-light photoreceptor system (FUN-LOV)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Vivid PAS domain (VVD)
Source of Species
Neurospora crassa
Class
LOV domain
UniProt ID
Q9C3Y6
GenBank
149243087
PDB ID
2PDR
3D Structure
Components
Photoreceptor: Vivid PAS domain (VVD)
Cofactor: Flavin adenine dinucleotide (FAD)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Fusion protein Regulatory protein GAL4
Detail Info
Recruited protein Regulatory protein Gal4
Detail Info
OS Application Information (1)
Mechanism of Action VVD-WC-1 heterodimerization [ 1]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application Optogenetic control of gene expression and flocculation in yeast
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Hours
Deactivation Time Hours
Light Intensity 100 micromoles per square centimeter per second
Expression Information Expression Method Transformation
Cell Lines Yeast
References
1 Fungal Light-Oxygen-Voltage Domains for Optogenetic Control of Gene Expression and Flocculation in Yeast