Details of the Controlled Protein

General Information of This Controlled Protein (CP)
CP ID CP00291
CP Name Virus protein 16 (VP16)
CP Type Fusion protein
Common Name Tegument protein VP16 (Alpha trans-inducing protein) (Alpha-TIF) (ICP25) (Vmw65)
Organism Human herpesvirus 1 (strain 17) (HHV-1) (Human herpes simplex virus 1)
UniProt ID P06492
UniProt Entry VP16_HHV11
KEGG vg:24271473
Function Transcriptional activator of immediate-early (IE) gene products (alpha genes). Acts as a key activator of lytic infection by initiating the lytic program through the assembly of the transcriptional regulatory VP16-induced complex composed of VP16 and two cellular factors, HCFC1 and POU2F 1. VP16-induced complex represents a regulatory switch: when it is on, it promotes IE-gene expression and thus lytic infection, and when it is off, it limits IE-gene transcription favoring latent infection.; May play a role in the aggregation of tegument proteins around nucleocapsids during virus morphogenesis.
Sequence
MDLLVDELFADMNADGASPPPPRPAGGPKNTPAAPPLYATGRLSQAQLMPSPPMPVPPAA
LFNRLLDDLGFSAGPALCTMLDTWNEDLFSALPTNADLYRECKFLSTLPSDVVEWGDAYV
PERTQIDIRAHGDVAFPTLPATRDGLGLYYEALSRFFHAELRAREESYRTVLANFCSALY
RYLRASVRQLHRQAHMRGRDRDLGEMLRATIADRYYRETARLARVLFLHLYLFLTREILW
AAYAEQMMRPDLFDCLCCDLESWRQLAGLFQPFMFVNGALTVRGVPIEARRLRELNHIRE
HLNLPLVRSAATEEPGAPLTTPPTLHGNQARASGYFMVLIRAKLDSYSSFTTSPSEAVMR
EHAYSRARTKNNYGSTIEGLLDLPDDDAPEEAGLAAPRLSFLPAGHTRRLSTAPPTDVSL
GDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGFTPHDSAPYGALDMADFEFEQMFT
DALGIDEYGG
Optogenetic System (OS) Controlling This CP
OS00047
OS Info
OS Name
Light activated rtTA system
[1]
Photoreceptor (PR) Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
PR Info
Components
Photoreceptor
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00520
OS Info
OS Name
Near-infrared light-controlled gene expression system
[2], [3]
Photoreceptor (PR) Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
PR Info
Components
Photoreceptor
Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein
Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor
Biliverdin (BV)
OS00598
OS Info
OS Name
Optogenetic activate gene expression system
[4]
Photoreceptor (PR) Name
Residue1-908 of Phytochrome B (PhyB (1-908))
PR Info
Components
Photoreceptor
Residue1-908 of Phytochrome B (PhyB (1-908))
Complementary Protein
Phytochrome interacting factor 6; PIF6 (PIF6)
Cofactor
Phycocyanobilin (PCB)
OS00525
OS Info
OS Name
Optogenetic control secreted alkaline phosphatase expression system
[5]
Photoreceptor (PR) Name
Phytochrome B (PhyB)
PR Info
Components
Photoreceptor
Phytochrome B (PhyB)
Complementary Protein
Phytochrome interacting factor 6 (PIF6)
Cofactor
Phycocyanobilin (PCB)
OS00526
OS Info
OS Name
Optogenetic control transcription activation system 1
[6]
Photoreceptor (PR) Name
Phytochrome B (PhyB)
PR Info
Components
Photoreceptor
Phytochrome B (PhyB)
Complementary Protein
Phytochrome interacting factor 6 (PIF6)
Cofactor
Phycocyanobilin (PCB)
OS00249
OS Info
OS Name
Optogenetic gene expression system
[7]
Photoreceptor (PR) Name
Erythrobacter litoralis 222 amino acid protein (EL222)
PR Info
Components
Photoreceptor
Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor
Flavin mononucleotide (FMN)
OS00014
OS Info
OS Name
Photoactivated split lexA transcriptional activation system
[8]
Photoreceptor (PR) Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
PR Info
Components
Photoreceptor
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
References
1 Optogenetic gene editing in regional skin
2 Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing
3 Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells
4 Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish
5 Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors
6 Optogenetics in Plants: Red/Far-Red Light Control of Gene Expression
7 An optogenetic gene expression system with rapid activation and deactivation kinetics
8 Optogenetic Control of Gene Expression in Drosophila