Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00520
OS Name
Near-infrared light-controlled gene expression system (NIR-GE)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Bacterial phytochrome photoreceptors 1 (Bphp1)
Source of Species
Rhodopseudomonas palustris
Class
Phytochromes
UniProt ID
A0A161I5N6
GenBank
1569047741
PDB ID
5OY5
3D Structure
Components
Photoreceptor: Bacterial phytochrome photoreceptors 1 (Bphp1)
Complementary Protein: Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor: Biliverdin (BV)
Complementary Protein Minimal BphP1-interacting module from PpsR2 with 17-kDa molecular mass (Q-PAS1)
Cofactor Biliverdin (BV)
Controlled Protein Recruited protein Regulatory protein Gal4
Detail Info
Fusion protein Virus protein 16
Detail Info
OS Application Information (1)
Mechanism of Action BphP1-Q-PAS1 heterodimerization [ 1] , [ 2]
Controlled Signal Pathway Transcription activation
Effect Activation
Application Light controlled transcription activation in mammalian cells
Light Information Activation Wavelength 740 nm
Process Reversible
Deactivation Wavelength 660 nm or Dark
Activation Time 30 s of light and 180 s of dark cycle for 3.5 h
Deactivation Time 4.4 min (dark); 2.3 min (620 nm)
Light Intensity 1 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells; U-2 OS cells; HEK293 cells; cos-7 cells;SH-SY5Y cells; Neuro-2a cells; NIH 3T3 cells
References
1 Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing
2 Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells