Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00525
|
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| OS Name |
Optogenetic control secreted alkaline phosphatase expression system
(Opto-SEAP)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Phytochrome interacting factor 6 (PIF6) | |||||
| Cofactor | Phycocyanobilin (PCB) | |||||
| Controlled Protein | Recruited protein | Secreted alkaline phosphatase | ||||
| Fusion protein | Virus protein 16 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | PhyB-PIF6 heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | Gene expression activation | |||||
| Effect | Activation | |||||
| Application | Red-far red light regulated gene expression provides an in cell readout of related PPI affinities | |||||
| Note | E means E-protein DNA-binding domain | |||||
| Light Information | Activation Wavelength | 660 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | 740 nm | |||||
| Activation Time | hours | |||||
| Deactivation Time | Hours | |||||
| Light Intensity | 20 micromoles per square meter per second | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | CHO K1 cells | |||||