Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00598
OS Name
Optogenetic activate gene expression system (Opto-AGE)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue1-908 of Phytochrome B (PhyB (1-908))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
Components
Photoreceptor: Residue1-908 of Phytochrome B (PhyB (1-908))
Complementary Protein: Phytochrome interacting factor 6; PIF6 (PIF6)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6; PIF6 (PIF6)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Fusion protein Tetracycline repressor protein class D
Detail Info
Virus protein 16
Detail Info
Recruited protein Tyrosine-protein kinase ZAP-70
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF3 heterodimerization [ 1]
Controlled Signal Pathway Gene expresion activation
Effect Activation
Application Red light regulated reversible nuclear localization of proteins to control gene expression in mammalian cells and zebrafish
Light Information Activation Wavelength 660 nm
Process Reversible
Deactivation Wavelength 740 nm
Activation Time 30 min
Deactivation Time 30 min
Light Intensity 20 micromoles per square meter
Expression Information Expression Method Transfection
Cell Lines CHO-K1 cells and Embryos cells
References
1 Red Light-Regulated Reversible Nuclear Localization of Proteins in Mammalian Cells and Zebrafish