Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00047
OS Name
Light activated rtTA system (Li-rtTA)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Source of Species
Arabidopsis thalino
Class
Cryptochromes
UniProt ID
Q96524
GenBank
NP_849588
PDB ID
6X24
3D Structure
Components
Photoreceptor: Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein: Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor: Flavin adenine dinucleotide (FAD)
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor Flavin adenine dinucleotide (FAD)
Controlled Protein Recruited protein Tetracycline repressor protein class D
Detail Info
Fusion protein Virus protein 16
Detail Info
OS Application Information (1)
Mechanism of Action CRY2-CIBN heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Optogenetic and doxycycline dural control gene editing in regional skin
Note Achieve induction efficiency with light illumination and dexycycline
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time 3 h
Deactivation Time Hours
Light Intensity 2.5 milliwatts per square centimeter or 5 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines keratinocytes
References
1 Optogenetic gene editing in regional skin