Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00127
PR Name Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species Erythrobacter litoralis
Class LOV domain
UniProt ID Q2NB98
GenBank 333944373
PDB ID 3P7N
Sequence
MRGSHHHHHHGMASMTGGQQMGRDLYDDDDKDHPFTMGQDRPIDGSGAPGADDTRVEVQP
PAQWVLDLIEASPIASVVSDPRLADNPLIAINQAFTDLTGYSEEECVGRNCRFLAGSGTE
PWLTDKIRQGVREHKPVLVEILNYKKDGTPFRNAVLVAPIYDDDDELLYFLGSQVEVDDD
QPNMGMARRERAAEMLKTLSPRQLEVTTLVASGLRNKEVAARLGLSEKTVKMHRGLVMEK
LNLKTSADLVRIAVEAGI
Components Photoreceptor Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor Flavin mononucleotide (FMN)
Detail Info
3D Structure
Click to Save PDB File
Cofactor Detail of This PR
Cofactor Name Flavin mononucleotide (FMN)
PubChem CID 643976
Structure
Formula C17H21N4O9P
Molecular Weight 456.3
Optogenetic System (OS) Consisting of This PR
OS00250
OS Info
OS Name: Blue light inducible promoter (PBLind-v1)
[1]
Fusion protein: RNA polymerase (RNAP) dependent promoter
Detail Info
OS00251
OS Info
OS Name: Blue light repressible promoter (PBLrep-v1)
[1]
Fusion protein: RNA polymerase
Detail Info
OS00256
OS Info
OS Name: Blue liight-responsive optogenetic OFF switch (Blue-OFF)
[2] , [3]
Fusion protein: KRAB domain of a transrepressor domain
Detail Info
OS00253
OS Info
OS Name: Enhenced blue light inducible promoter vision 1 (PEBLindv1)
[4] , [1]
Functional protein: Erythrobacter litoralis 222 amino acid protein
Detail Info
OS00254
OS Info
OS Name: Enhenced blue light inducible promoter vision 2 (PEBLindv2)
[4] , [1]
Functional protein: Erythrobacter litoralis 222 amino acid protein
Detail Info
OS00257
OS Info
OS Name: Light controlled exopolysaccharide expression system (LCEPSE)
[5]
Fusion protein: EPS production protein
Detail Info
OS00258
OS Info
OS Name: Light controlled sfGFP expression system (LCsfGFPE)
[5]
Functional protein: Erythrobacter litoralis 222 amino acid protein
Detail Info
OS00259
OS Info
OS Name: Light-regulated stochastic transcriptional regulation system (LRSTR)
[6]
Functional protein: Erythrobacter litoralis 222 amino acid protein
Detail Info
OS00252
OS Info
OS Name: Optogenetic CRISPR interference system (Opto-CRISPRi)
[7]
Functional protein: EL222 operator
Detail Info
OS00249
OS Info
OS Name: Optogenetic gene expression system (Opto-GE)
[8]
Fusion protein: Virus protein 16
Detail Info
OS00255
OS Info
OS Name: Zebrafish-optimized optogenetic gene expression system (TAEL)
[9] , [10]
Fusion protein: An optimized Gal4-activator
Detail Info
References
1 Blue light-mediated transcriptional activation and repression of gene expression in bacteria
2 Dual-controlled optogenetic system for the rapid down-regulation of protein levels in mammalian cells
3 Silencing of RNA polymerases II and III-dependent transcription by the KRAB protein domain of KOX1, a Krppel-type zinc finger factor
4 Cell-Free Optogenetic Gene Expression System
5 Optogenetics in Sinorhizobium meliloti Enables Spatial Control of Exopolysaccharide Production and Biofilm Structure
6 An Optogenetic Platform for Real-Time, Single-Cell Interrogation of Stochastic Transcriptional Regulation
7 Engineering an Optogenetic CRISPRi Platform for Improved Chemical Production
8 An optogenetic gene expression system with rapid activation and deactivation kinetics
9 TAEL: a zebrafish-optimized optogenetic gene expression system with fine spatial and temporal control
10 Optimized Gal4 genetics for permanent gene expression mapping in zebrafish