Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00568
|
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| OS Name |
Ultra-light-sensitive optogenetic Ca2+ modulator
(MonSTIM1)
|
[1] | ||||
|
Photoreceptor (PR) |
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| Complementary Protein | Constitutive photomorphogenic 1 (COP1) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | Stromal interaction molecule 1 | ||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2 homo-oligomerization | [ 1] | ||||
| Controlled Signal Pathway | CRAC channel activation and Ca2+ influx | |||||
| Effect | Activation | |||||
| Application | Optogenetic control endogenous Ca2+ channel in awake mice | |||||
| Note | High propensity to achieve advanced oligomeric state in the basal condition | |||||
| Light Information | Activation Wavelength | 473 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 62 s | |||||
| Deactivation Time | 7min | |||||
| Light Intensity | 1 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transduction | ||||
| Cell Lines | Mouse excitatory neurons or astrocytes | |||||