Details of the Optogenetic Tool

General Information of This Photoreceptor (PR)
PR ID PR00009
PR Name Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G))
Source of Species Arabidopsis thalino
Class Cryptochromes
UniProt ID Q96524
GenBank NP_849588
PDB ID 6K8I
Mutation Site E490G
Sequence
MKMDKKTIVWFRRDLRIEDNPALAAAAHEGSVFPVFIWCPEEEGQFYPGRASRWWMKQSL
AHLSQSLKALGSDLTLIKTHNTISAILDCIRVTGATKVVFNHLYDPVSLVRDHTVKEKLV
ERGISVQSYNGDLLYEPWEIYCEKGKPFTSFNSYWKKCLDMSIESVMLPPPWRLMPITAA
AEAIWACSIEELGLENEAEKPSNALLTRAWSPGWSNADKLLNEFIEKQLIDYAKNSKKVV
GNSTSLLSPYLHFGEISVRHVFQCARMKQIIWARDKNSEGEESADLFLRGIGLREYSRYI
CFNFPFTHEQSLLSHLRFFPWDADVDKFKAWRQGRTGYPLVDAGMRELWATGWMHNRIRV
IVSSFAVKFLLLPWKWGMKYFWDTLLDADLECDILGWQYISGSIPDGHELDRLDNPALQG
AKYDPEGEYIRQWLPELARLPTEWIHHPWDAPLTVLKASGVELGTNYAKPIVDIDTAREL
LAKAISRTREAQIMIGAAPDEIVADSFEALGANTIKEPGLCPSVSSNDQQVPSAVRYNGS
KRVKPEEEEERDMKKSRGFDERELFSTAESSSSSSVFFVSQSCSLASEGKNLEGIQDSSD
QITTSLGKNGCK
Components Photoreceptor Photolyase homology region of Cryptochromes 2 (E490G) (CRY2PHR (E490G))
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Detail Info
Cofactor Flavin adenine dinucleotide (FAD)
Detail Info
3D Structure
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Complementary Protein Detail of This PR
Complementary Protein Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
UniProt ID A0A061DZ54
Sequence
MNRALPEMLQCSDMTVLERQRARLKWQQEQLQQQQLQQQEQQQSYFSELSGVFSSQPSHV
EGFQGGLMSGDSVLGDMVMTRQLKPDPGLETAWPELVKVDMPGMGFGPCGYGNGPSFDMN
YAISRTSSCPPAVAAAVAGEVVEVKGKESVVSEKIGSAVGRESFKKRKVDKLQNLKVVAE
DDSKRIKACAEEGESKITGPNTNKSSSNNNNNKKESSTDTSKENSKVSEVQKPDYIHVRA
RRGQATDSHSLAERVRREKISERMKYLQDLVPGCNKITGKAGMLDEIINYVQSLQRQVEF
LSMKLAAVNPRLDFNVENLFAKEVFPSCTTNFPTVGMSSEMANPPYLQVSPVQHVVSCCG
LEMGMNTPDMAPRRTISAPVSIPDASFLDSSCFPQIQPSATWDVELQNLYNVAFDQGRST
SFPSQPFTGSIEASNLKMEM
Cofactor Detail of This PR
Cofactor Name Flavin adenine dinucleotide (FAD)
PubChem CID 643975
Structure
Formula C27H33N9O15P2
Molecular Weight 785.5
Optogenetic System (OS) Consisting of This PR
OS00070
OS Info
OS Name: Enhenced optogentic regulate stromal interaction molecule 1 system 2 (EOptoSTIM1-2)
[1]
Fusion protein: Stromal interaction molecule 1
Detail Info
OS00071
OS Info
OS Name: Enhenced optogentic regulate stromal interaction molecule 1 system 3 (EOptoSTIM1-3)
[1]
Fusion protein: Stromal interaction molecule 1
Detail Info
OS00069
OS Info
OS Name: Light-Induced Co-clustering (LINC)
[2]
Fusion protein: Clathrin light chain
Detail Info
References
1 Optogenetic manipulation of calcium signals in single T cells in vivo
2 An optimized optogenetic clustering tool for probing protein interaction and function