Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00177
OS Name
Optogentic regulate stromal interaction molecule 1 system 5 (OptoSTIM1-5)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Improved Light-Inducible Dimer (iLID)
Source of Species
Avena sativa
Class
LOV domain
UniProt ID
O49003
GenBank
747155673
PDB ID
4WF0
3D Structure
Components
Photoreceptor: Improved Light-Inducible Dimer (iLID)
Complementary Protein: Stringent starvation protein B (SspB)
Cofactor: Flavin mononucleotide (FMN)
Complementary Protein Stringent starvation protein B (SspB)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Recruited protein Stringent starvation protein B
Detail Info
Fusion protein Stromal interaction molecule 1
Detail Info
OS Application Information (1)
Mechanism of Action ILID comformational change and subsequently iLID-SspB heterodimerization [ 1]
Controlled Signal Pathway STIM1-gated Ca2+ influxion
Effect Activation
Application STIM1-gated Ca2+ influx to probe the molecular choreography
Note STIM1 is a stromal interaction molecule
Light Information Activation Wavelength 470 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time 48.6 ± 5.4 s
Light Intensity 4.0 milliwatts per square centimeter
Expression Information Expression Method Transfection
Cell Lines Hela cells
References
1 Optogenetic engineering to probe the molecular choreography of STIM1-mediated cell signaling