Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00541
OS Name
Optogenetic-drived affinity purification system (Opto-DAP)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue1-650 of Phytochrome B (PhyB (1-650))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Residue1-650 of Phytochrome B (PhyB (1-650))
Complementary Protein: Phytochrome interacting factor 6 (PIF6)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6 (PIF6)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Functional protein Phytochrome B
Detail Info
Recruited protein Tyrosine-protein kinase ZAP-70
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF6 heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Light controlled affinity purification of protein complexes
Note PIF concentration affects purification efficiency and the system can be used to purify other protein of interest
Light Information Activation Wavelength 660 nm
Process Reversible
Deactivation Wavelength 740 nm
Activation Time 60 min
Deactivation Time 10 min
Light Intensity 10 micromoles per square meter per second (660nm); 70 micromoles per square meter per second (740nm)
Expression Information Expression Method Transduction
Cell Lines Jurkat cell line P116
References
1 Light-Controlled Affinity Purification of Protein Complexes Exemplified by the Resting ZAP70 Interactome