Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00542
OS Name
Optogenetic bind and release biomolecules trap (Opto-trap)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue1-650 of Phytochrome B (PhyB (1-650))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Residue1-650 of Phytochrome B (PhyB (1-650))
Complementary Protein: Phytochrome interacting factor 6 (PIF6)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 6 (PIF6)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Functional protein Phytochrome B
Detail Info
Recruited protein Phytochrome interacting factor 3
Detail Info
Phytochrome interacting factor 6
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF6 heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Sensor
Application Spatiotemporally remote-control the release of target molecules into environment
Note Release molecule under exposure of 740nm
Light Information Activation Wavelength 660 nm
Process Reversible
Deactivation Wavelength 740 nm
Activation Time 5 min
Deactivation Time 5 min
Light Intensity 100 micromoles per square meter per second (660nm) 110 micromoles per square meter per second (740nm)
Expression Information Expression Method Transfection
Cell Lines HEK293T cells
References
1 Generic and reversible opto-trapping of biomolecules