Details of the Optogenetic Tool | OPTICS

General Information of This Photoreceptor (PR)
PR ID	PR00304
PR Name	Residue1-621 of Phytochrome B (PhyB (1-621))
Source of Species	Arabidopsis
Class	Phytochromes
UniProt ID	P14713
GenBank	NP_179469
PDB ID	4OUR
Sequence	MVSGVGGSGGGRGGGRGGEEEPSSSHTPNNRRGGEQAQSSGTKSLRPRSNTESMSKAIQQ YTVDARLHAVFEQSGESGKSFDYSQSLKTTTYGSSVPEQQITAYLSRIQRGGYIQPFGCM IAVDESSFRIIGYSENAREMLGIMPQSVPTLEKPEILAMGTDVRSLFTSSSSILLERAFV AREITLLNPVWIHSKNTGKPFYAILHRIDVGVVIDLEPARTEDPALSIAGAVQSQKLAVR AISQLQALPGGDIKLLCDTVVESVRDLTGYDRVMVYKFHEDEHGEVVAESKRDDLEPYIG LHYPATDIPQASRFLFKQNRVRMIVDCNATPVLVVQDDRLTQSMCLVGSTLRAPHGCHSQ YMANMGSIASLAMAVIINGNEDDGSNVASGRSSMRLWGLVVCHHTSSRCIPFPLRYACEF LMQAFGLQLNMELQLALQMSEKRVLRTQTLLCDMLLRDSPAGIVTQSPSIMDLVKCDGAA FLYHGKYYPLGVAPSEVQIKDVVEWLLANHADSTGLSTDSLGDAGYPGAAALGDAVCGMA VAYITKRDFLFWFRSHTAKEIKWGGAKHHPEDKDDGQRMHPRSSFQAFLEVVKSRSQPWE TAEMDAIHSLQLILRDSFKES
Components	Photoreceptor	Residue1-621 of Phytochrome B (PhyB (1-621))
	Complementary Protein	Phytochrome interacting factor 3 (PIF3)				Detail Info
	Cofactor	Phycocyanobilin (PCB)				Detail Info
3D Structure
3D Structure	Click to Save PDB File

Complementary Protein Detail of This PR
Complementary Protein	Phytochrome interacting factor 3 (PIF3)
UniProt ID	O80536
Sequence	MPLFELFRLTKAKLESAQDRNPSPPVDEVVELVWENGQISTQSQSSRSRNIPPPQANSSR AREIGNGSKTTMVDEIPMSVPSLMTGLSQDDDFVPWLNHHPSLDGYCSDFLRDVSSPVTV NEQESDMAVNQTAFPLFQRRKDGNESAPAASSSQYNGFQSHSLYGSDRARDLPSQQTNPD RFTQTQEPLITSNKPSLVNFSHFLRPATFAKTTNNNLHDTKEKSPQSPPNVFQTRVLGAK DSEDKVLNESVASATPKDNQKACLISEDSCRKDQESEKAVVCSSVGSGNSLDGPSESPSL SLKRKHSNIQDIDCHSEDVEEESGDGRKEAGPSRTGLGSKRSRSAEVHNLSERRRRDRIN EKMRALQELIPNCNKVDKASMLDEAIEYLKSLQLQVQIMSMASGYYLPPAVMFPPGMGHY PAAAAAMAMGMGMPYAMGLPDLSRGGSSVNHGPQFQVSGMQQQPVAMGIPRVSGGGIFAG SSTIGNGSTRDLSGSKDQTTTNNNSNLKPIKRKQGSSDQFCGSS

Cofactor Detail of This PR
Cofactor Name	Phycocyanobilin (PCB)
PubChem CID	5280816
Structure
Formula	C33H38N4O6
Molecular Weight	586.7

Optogenetic System (OS) Consisting of This PR

OS00532

OS Name: Light-induced dimerization system 2 (LID 2)

[1] , [2]

Fusion protein: C terminal of GTPase HRas

Recruited protein: Tyrosine-protein kinase ZAP-70

OS00533

OS Name: Light-induced dimerization system 3 (LID 3)

[1] , [2]

Recruited protein: Endogenous Rac1 protein

Functional protein: Phytochrome B

OS00534

OS Name: Light-induced dimerization system 4 (LID 4)

[1] , [2]

Functional protein: Phytochrome B

Recruited protein: Protein kinase CRAF

OS00529

OS Name: Optogenetic control heterologous metabolism system (Opto-HM)

[3]

Fusion protein: C terminal of T7 RNA polymerase

Recruited protein: T7 RNA polymerase

OS00531

OS Name: Optogenetic control protein location system based on PhyB (Opto-CPL-PhyB)

[4]

Functional protein: Phytochrome B

Recruited protein: Phytochrome interacting factor 6

OS00530

OS Name: Optogenetic control SOS activity system (Opto-SOS)

[5]

Recruited protein: Nucleotide exchange factor SOS

Functional protein: Phytochrome B

OS00535

OS Name: Optogenetic control transcription activation system 2 (Opto-CTA 2)

[6]

Recruited protein: Activation domain

Fusion protein: DNA Binding Domain

References

1	Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling
2	Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics
3	Optogenetic Control of Heterologous Metabolism in E. coli
4	Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors
5	Optogenetic Control of Ras/Erk Signaling Using the Phy-PIF System
6	Biosynthesis of Orthogonal Molecules Using Ferredoxin and Ferredoxin-NADP(+) Reductase Systems Enables Genetically Encoded PhyB Optogenetics