Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00530
OS Name
Optogenetic control SOS activity system (Opto-SOS)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue1-621 of Phytochrome B (PhyB (1-621))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Residue1-621 of Phytochrome B (PhyB (1-621))
Complementary Protein: Phytochrome interacting factor 3 (PIF3)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 3 (PIF3)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Recruited protein Nucleotide exchange factor SOS
Detail Info
Functional protein Phytochrome B
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF6 heterodimerization [ 1]
Controlled Signal Pathway Control Ras/Erk signaling
Effect Sensor
Application Optocontrol Ras/Erk signaling using the Phy-PIF system
Note Interaction persists for hours in the absence of light input
Light Information Activation Wavelength 650 nm
Process Reversible
Deactivation Wavelength 750 nm
Activation Time Seconds
Deactivation Time Seconds
Light Intensity 20 micromoles per square meter per second(650nm) 300 micromoles per square meter per second (750nm)
Expression Information Expression Method Transfection and Transduction
Cell Lines HEK 293T LX cells
References
1 Optogenetic Control of Ras/Erk Signaling Using the Phy-PIF System