Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID	OS00532
OS Name	Light-induced dimerization system 2 (LID 2)		[1], [2]
Photoreceptor (PR) PR Info	PR Name Residue1-621 of Phytochrome B (PhyB (1-621)) Source of Species Arabidopsis Class Phytochromes UniProt ID P14713 GenBank NP_179469 PDB ID 4OUR 3D Structure Components Photoreceptor: Residue1-621 of Phytochrome B (PhyB (1-621)) Complementary Protein: Phytochrome interacting factor 3 (PIF3) Cofactor: Phycocyanobilin (PCB)
Complementary Protein	Phytochrome interacting factor 3 (PIF3)
Cofactor	Phycocyanobilin (PCB)
Controlled Protein	Fusion protein	C terminal of GTPase HRas	Detail Info
Controlled Protein	Recruited protein	Tyrosine-protein kinase ZAP-70	Detail Info

OS Application Information (1)
Mechanism of Action	PhyB-PIF3/PIF6 heterodimerization		[ 1] , [ 2]
Controlled Signal Pathway	Protein translocation
Effect	Activation
Application	Optogenetic contol of cell signal by the PhyB-PIF system in the absence of exogenous chromophores
Note	PCB was synthesised in mammalian cells
Light Information	Activation Wavelength	625 nm
	Process	Reversible
	Deactivation Wavelength	730 nm
	Activation Time	Seconds to Minutes
	Deactivation Time	Seconds to Minutes
Expression Information	Expression Method	Transfection
Expression Information	Cell Lines	HEK293 cells

References

1	Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling
2	Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics