Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00531
OS Name
Optogenetic control protein location system based on PhyB (Opto-CPL-PhyB)
 [1]
Photoreceptor (PR)
PR Info
PR Name
Residue1-621 of Phytochrome B (PhyB (1-621))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Residue1-621 of Phytochrome B (PhyB (1-621))
Complementary Protein: Phytochrome interacting factor 3 (PIF3)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 3 (PIF3)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Functional protein Phytochrome B
Detail Info
Recruited protein Phytochrome interacting factor 6
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF6 heterodimerization [ 1]
Controlled Signal Pathway Protein translocation
Effect Activation
Application Light induced recruitment of target protein to the plasma membrane
Light Information Activation Wavelength 635 nm
Process Reversible
Deactivation Wavelength 730 nm
Activation Time 1 min
Deactivation Time Minutes
Light Intensity 50 milliwatts per square centimeter(635nm) 10 milliwatts per square centimeter(730nm)
Expression Information Expression Method Transduction
Cell Lines NIH 3T3 cells
References
1 Deconstructing and repurposing the light-regulated interplay between Arabidopsis phytochromes and interacting factors