Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00533
OS Name
Light-induced dimerization system 3 (LID 3)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Residue1-621 of Phytochrome B (PhyB (1-621))
Source of Species
Arabidopsis
Class
Phytochromes
UniProt ID
P14713
GenBank
NP_179469
PDB ID
4OUR
3D Structure
Components
Photoreceptor: Residue1-621 of Phytochrome B (PhyB (1-621))
Complementary Protein: Phytochrome interacting factor 3 (PIF3)
Cofactor: Phycocyanobilin (PCB)
Complementary Protein Phytochrome interacting factor 3 (PIF3)
Cofactor Phycocyanobilin (PCB)
Controlled Protein Recruited protein Endogenous Rac1 protein
Detail Info
Functional protein Phytochrome B
Detail Info
OS Application Information (1)
Mechanism of Action PhyB-PIF3/PIF6 heterodimerization [ 1] , [ 2]
Controlled Signal Pathway Rac1 activation
Effect Activation
Application Translocate Tiam1 to plasma membrane to manipulate signal transduction
Note PCB was synthesised in mammalian cells
Light Information Activation Wavelength 625 nm
Process Reversible
Deactivation Wavelength 730 nm
Activation Time Seconds to Minutes
Deactivation Time Seconds to Minutes
Expression Information Expression Method Transfection
Cell Lines HEK293 cells
References
1 Efficient synthesis of phycocyanobilin in mammalian cells for optogenetic control of cell signaling
2 Improvement of Phycocyanobilin Synthesis for Genetically Encoded Phytochrome-Based Optogenetics