Details of the Optogenetic System

General Information of This Optogenetic System (OS)
OS ID
OS00253
OS Name
Enhenced blue light inducible promoter vision 1 (PEBLindv1)
 [1], [2]
Photoreceptor (PR)
PR Info
PR Name
Erythrobacter litoralis 222 amino acid protein (EL222)
Source of Species
Erythrobacter litoralis
Class
LOV domain
UniProt ID
Q2NB98
GenBank
333944373
PDB ID
3P7N
3D Structure
Components
Photoreceptor: Erythrobacter litoralis 222 amino acid protein (EL222)
Cofactor: Flavin mononucleotide (FMN)
Cofactor Flavin mononucleotide (FMN)
Controlled Protein Functional protein Erythrobacter litoralis 222 amino acid protein
Detail Info
OS Application Information (1)
Mechanism of Action EL222 homodimerization and DNA binding [ 1] , [ 2]
Controlled Signal Pathway Gene expression activation
Effect Activation
Application A high-throughput screening technology for rapid prototyping of complex biological networks in both space and time
Note Designed from one component-based blue light-inducible Escherichia coli promoter; Promotor: J23100: strong promoter; B0015: terminator; rbs34: default ribosome binding site
Light Information Activation Wavelength 460 nm
Process Reversible
Deactivation Wavelength Dark
Activation Time Seconds
Deactivation Time Seconds
Light Intensity 12 watts per square meter
References
1 Cell-Free Optogenetic Gene Expression System
2 Blue light-mediated transcriptional activation and repression of gene expression in bacteria