Details of the Controlled Protein

General Information of This Controlled Protein (CP)
CP ID CP00304
CP Name Regulatory protein GAL4 (Gal4BD)
CP Type Fusion protein
Common Name Regulatory protein GAL4
Organism Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
UniProt ID P04386
UniProt Entry GAL4_YEAST
KEGG sce:YPL248C
Function This protein is a positive regulator for the gene expression of the galactose-induced genes such as GAL1, GAL2, GAL7, GAL10, and MEL1 which code for the enzymes used to convert galactose to glucose. It recognizes a 17 base pair sequence in (5'-CGGRNNRCYNYNCNCCG-3') the upstream activating sequence (UAS-G) of these genes.
Sequence
MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTKRSPLTRAHLTEVESR
LERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNKDAVTDRLASVETDMPL
TLRQHRISATSSSEESSNKGQRQLTVSIDSAAHHDNSTIPLDFMPRDALHGFDWSEEDDM
SDGLPFLKTDPNNNGFFGDGSLLCILRSIGFKPENYTNSNVNRLPTMITDRYTLASRSTT
SRLLQSYLNNFHPYCPIVHSPTLMMLYNNQIEIASKDQWQILFNCILAIGAWCIEGESTD
IDVFYYQNAKSHLTSKVFESGSIILVTALHLLSRYTQWRQKTNTSYNFHSFSIRMAISLG
LNRDLPSSFSDSSILEQRRRIWWSVYSWEIQLSLLYGRSIQLSQNTISFPSSVDDVQRTT
TGPTIYHGIIETARLLQVFTKIYELDKTVTAEKSPICAKKCLMICNEIEEVSRQAPKFLQ
MDISTTALTNLLKEHPWLSFTRFELKWKQLSLIIYVLRDFFTNFTQKKSQLEQDQNDHQS
YEVKRCSIMLSDAAQRTVMSVSSYMDNHNVTPYFAWNCSYYLFNAVLVPIKTLLSNSKSN
AENNETAQLLQQINTVLMLLKKLATFKIQTCEKYIQVLEEVCAPFLLSQCAIPLPHISYN
NSNGSAIKNIVGSATIAQYPTLPEENVNNISVKYVSPGSVGPSPVPLKSGASFSDLVKLL
SNRPPSRNSPVTIPRSTPSHRSVTPFLGQQQQLQSLVPLTPSALFGGANFNQSGNIADSS
LSFTFTNSSNGPNLITTQTNSQALSQPIASSNVHDNFMNNEITASKIDDGNNSKPLSPGW
TDQTAYNAFGITTGMFNTTTMDDVYNYLFDDEDTPPNPKKE
Optogenetic System (OS) Controlling This CP
OS00149
OS Info
OS Name
Blue light-controlled gene expression system
[1], [2]
Photoreceptor (PR) Name
Vivid PAS domain (VVD)
PR Info
Components
Photoreceptor
Vivid PAS domain (VVD)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00565
OS Info
OS Name
Irreversible Photoactivated Gal4 trancription system
[3], [4], [5]
Photoreceptor (PR) Name
Flavin Kelch-repeat F-box 1 (FKF1)
PR Info
Components
Photoreceptor
Flavin Kelch-repeat F-box 1 (FKF1)
Complementary Protein
GIGANTEA (GI)
Cofactor
Flavin mononucleotide (FMN)
OS00053
OS Info
OS Name
Light-triggered modulation of K+ conductance system in mitochondria
[6]
Photoreceptor (PR) Name
Cryptochrome 2 (CRY2)
PR Info
Components
Photoreceptor
Cryptochrome 2 (CRY2)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00150
OS Info
OS Name
Optogenetic control gene expression by two LOV blue-light photoreceptor system
[7]
Photoreceptor (PR) Name
Vivid PAS domain (VVD)
PR Info
Components
Photoreceptor
Vivid PAS domain (VVD)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00537
OS Info
OS Name
Photoactivable Gal4 for gene expression control system
[8]
Photoreceptor (PR) Name
Residue1-650 of Phytochrome B (PhyB (1-650))
PR Info
Components
Photoreceptor
Residue1-650 of Phytochrome B (PhyB (1-650))
Complementary Protein
Phytochrome interacting factor 6 (PIF6)
Cofactor
Phycocyanobilin (PCB)
OS00566
OS Info
OS Name
Photoactivable Gal4 for gene expression controlled by truncated PhyB system
[9]
Photoreceptor (PR) Name
Residue 1-450 of Phytochrome B (PhyB (1-450))
PR Info
Components
Photoreceptor
Residue 1-450 of Phytochrome B (PhyB (1-450))
Complementary Protein
Phytochrome interacting factor 6 (C9S/C10S/S37A) (PIFS=PIF6(C9S/C10S/S37A))
Cofactor
Phycocyanobilin (PCB)
OS00515
OS Info
OS Name
Photoactivatable GAL promoter to control transcription
[10]
Photoreceptor (PR) Name
Phytochrome B (PhyB)
PR Info
Components
Photoreceptor
Phytochrome B (PhyB)
Complementary Protein
Phytochrome interacting factor 3 (PIF3)
Cofactor
Phycocyanobilin (PCB)
OS00031
OS Info
OS Name
Photoactivatable GAL promoter to control transcription system 2
[]
Photoreceptor (PR) Name
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
PR Info
Components
Photoreceptor
Photolyase homology region of Arabidopsis Cryptochromes 2 (CRY2PHR)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
OS00059
OS Info
OS Name
Photoactivatable GAL promoter to control transcription system 3
[11]
Photoreceptor (PR) Name
Cryptochrome 2 (CRY2)
PR Info
Components
Photoreceptor
Cryptochrome 2 (CRY2)
Complementary Protein
Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN)
Cofactor
Flavin adenine dinucleotide (FAD)
References
1 Near-infrared light-controlled systems for gene transcription regulation, protein targeting and spectral multiplexing
2 Near-Infrared Light-Controlled Gene Expression and Protein Targeting in Neurons and Non-neuronal Cells
3 Optimized light-inducible transcription in mammalian cells using Flavin Kelch-repeat F-box1/GIGANTEA and CRY2/CIB1
4 Induction of protein-protein interactions in live cells using light
5 FKF1 and GIGANTEA complex formation is required for day-length measurement in Arabidopsis
6 Light-Regulated Transcription of a Mitochondrial-Targeted K(+) Channel
7 Fungal Light-Oxygen-Voltage Domains for Optogenetic Control of Gene Expression and Flocculation in Yeast
8 PhotoGal4: A Versatile Light-Dependent Switch for Spatiotemporal Control of Gene Expression in Drosophila Explants
9 Light-controllable Transcription System by Nucleocytoplasmic Shuttling of a Truncated Phytochrome B
10 Light-mediated control of DNA transcription in yeast
11 Real-time optogenetic control of intracellular protein concentration in microbial cell cultures