Details of the Optogenetic System
| General Information of This Optogenetic System (OS) | ||||||
|---|---|---|---|---|---|---|
| OS ID |
OS00053
|
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| OS Name |
Light-triggered modulation of K+ conductance system in mitochondria
(LTMK+ in Mito)
|
[1] | ||||
|
Photoreceptor (PR) |
|
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| Complementary Protein | Cryptochrome-interacting basic-helix-loop-helix 1 (CIB1/CIBN) | |||||
| Cofactor | Flavin adenine dinucleotide (FAD) | |||||
| Controlled Protein | Fusion protein | Regulatory protein GAL4 | ||||
| Recruited protein | Virus protein 16 | |||||
| OS Application Information (1) | ||||||
|---|---|---|---|---|---|---|
| Mechanism of Action | CRY2-CIBN heterodimerization | [ 1] | ||||
| Controlled Signal Pathway | K+ channel transcription in mitochondria | |||||
| Effect | Activation | |||||
| Application | Optogenetic control transcription of a mitochondrial targeted K+ channel | |||||
| Note | Gene of interest (GOI) including N-terminal of GalBS and C-terminal of the GFP tag was cloned downstream of CRY2-CIB1 coding region | |||||
| Light Information | Activation Wavelength | 450 nm | ||||
| Process | Reversible | |||||
| Deactivation Wavelength | Dark | |||||
| Activation Time | 1 h | |||||
| Deactivation Time | Hours | |||||
| Light Intensity | 0.16 milliwatts per square centimeter | |||||
| Expression Information | Expression Method | Transfection | ||||
| Cell Lines | HEK293 cells and Cos7 cells | |||||